Identification of QTLs and a Candidate Gene for Reducing Pre-Harvest Sprouting in Aegilops tauschii–Triticum aestivum Chromosome Segment Substitution Lines
| العنوان: | Identification of QTLs and a Candidate Gene for Reducing Pre-Harvest Sprouting in Aegilops tauschii–Triticum aestivum Chromosome Segment Substitution Lines |
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| المؤلفون: | Xian Chen, Yarui Su, Minjie Hu, Qing Su, Suoping Li, Shaoguang Sun, Yuge Li, Dale Zhang, Jie He |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 3729, p 3729 (2021) International Journal of Molecular Sciences Volume 22 Issue 7 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Candidate gene, Aegilops, Quantitative Trait Loci, quantitative trait loci (QTLs), Single-nucleotide polymorphism, Germination, Biology, Quantitative trait locus, Real-Time Polymerase Chain Reaction, 01 natural sciences, Catalysis, Homology (biology), Article, Inorganic Chemistry, lcsh:Chemistry, 03 medical and health sciences, RNA-Sequence, Aegilops tauschii, Physical and Theoretical Chemistry, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, Triticum, Genetics, Sequence Analysis, RNA, Kompetitive allele-specific PCR (KASP), Organic Chemistry, Chromosome, Kompetitive Allele Specific PCR (KASP), qRT-PCR, General Medicine, pre-harvest sprouting (PHS), biology.organism_classification, Computer Science Applications, chromosome segment substitution lines (CSSL), 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Microsatellite, 010606 plant biology & botany |
| الوصف: | Wheat pre-harvest sprouting (PHS) causes serious losses in wheat yield. In this study, precise mapping was carried out in the chromosome segment substitution lines (CSSL) F2 population generated by a direct cross of Zhoumai 18 (PHS-sensitive) and Aegilops tauschii accession T093 (highly PHS-resistant). Three Ae. tauschii-derived quantitative trait loci (QTLs), QDor.3D.1, QDor.3D.2, and QDor.3D.3, were detected on chromosome 3DL using four simple sequence repeats (SSR) markers and 10 developed Kompetitive allele-specific PCR (KASP) markers. Alongside these QTL results, the RNA-Seq and qRT-PCR analysis revealed expression levels of TraesCS3D01G466100 in the QDor.3D.2 region that were significantly higher in CSSLs 495 than in Zhoumai 18 during the seed imbibition treatment. The cDNA sequencing results of TraesCS3D01G466100 showed two single nucleotide polymorphisms (SNPs), resulting in two changed amino acid substitutions between Zhoumai 18 and line 495, and the 148 nt amino acid substitution of TraesCS3D01G466100, derived from Ae. tauschii T093, which may play an important role in the functioning of ubiquitin ligase enzymes 3 (E3) according to the homology protein analysis, which could lead to differential PHS-resistance phenotypes. Taken together, our results may foster a better understanding of the mechanism of PHS resistance and are potentially valuable for marker-assisted selection in practical wheat breeding efforts. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad5e9234a38f0a31f60dae9909101609 https://www.mdpi.com/1422-0067/22/7/3729 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ad5e9234a38f0a31f60dae9909101609 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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