Insulin-like growth factor binding proteins in sera of pregnant nonhuman primates
| العنوان: | Insulin-like growth factor binding proteins in sera of pregnant nonhuman primates |
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| المؤلفون: | Asgerally T. Fazleabas, Sharron E. Gargosky, Linda C. Giudice, Richard L. Stouffer, B A Dsupin, M B Zelinski-Wooten, Ron G. Rosenfeld, L. de las Fuentes |
| المصدر: | Endocrinology. 132:1514-1526 |
| بيانات النشر: | The Endocrine Society, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | medicine.medical_specialty, medicine.medical_treatment, Blotting, Western, Insulin-like growth factor-binding protein, Endocrinology, Pregnancy, Somatomedins, Internal medicine, Endopeptidases, medicine, Animals, Humans, Immunoassay, Gel electrophoresis, Fetus, Protease, biology, medicine.diagnostic_test, Proteolytic enzymes, Macaca mulatta, Precipitin Tests, Somatomedin, Insulin-Like Growth Factor Binding Proteins, biology.protein, Pregnancy, Animal, Female, Carrier Proteins, hormones, hormone substitutes, and hormone antagonists, Postpartum period, Papio |
| الوصف: | Insulin-like growth factors (IGFs) are mitogenic peptides that are important for fetal and maternal tissue growth during pregnancy. They circulate complexed primarily with a serum binding protein, IGFBP-3, which regulates the availability of the IGFs to their target tissues. We previously reported that in pregnant women, serum IGFBP-3 levels, assessed by Western ligand blotting, decline markedly beginning at 6 weeks gestation due to a circulating protease that cleaves IGFBP-3 into a 29-kilodalton (kDa) protein and lower mol wt (M(r)) fragments. In the current study, we compared IGFBP profiles, IGFBP-3 and IGFBP-1 levels, and IGFBP protease activities in sera from pregnant and nonpregnant women, baboons, and rhesus monkeys, using Western ligand blotting, IGFBP-specific immunoassays, IGFBP-3 protease assay, and zymographic gel electrophoresis. Serum IGFBP profiles in nonpregnant human and nonhuman primates were similar and were not cycle-dependent. IGFBP-3 (37-43 kDa), IGFBP-2 (31 kDa), and IGFBP-1 (28 kDa) were identified in all three species using IGFBP-specific human antisera. A 24-kDa IGFBP was also present and is believed to be IGFBP-4. Serum IGFBP-1 levels increased throughout gestation in human and nonhuman primates. Serum IGFBP-2 and putative IGFBP-4 were barely detectable in all three species from midgestation to term, but increased several days postpartum. In contrast, serum IGFBP-3 profiles differed markedly between species during gestation. Rather than the decrease seen in human pregnancy serum, there was an increase in circulating IGFBP-3 levels in nonhuman primates. Furthermore, for both baboon and rhesus monkey, the M(r) of serum IGFBP-3 was about 2 kDa greater in pregnant than in nonpregnant animals, and deglycosylation studies suggested that the higher M(r) forms may be alternatively glycosylated or may have a unique primary structure. As in nonpregnant women, serum IGFBP-3 protease activity was absent in nonpregnant and pregnant baboons. However, rhesus monkey serum contained a calcium-dependent protease that cleaved recombinant human IGFBP-3 into unique fragments, compared to the human pregnancy enzyme. Unlike human pregnancy serum, which proteolyzes IGFBP-3, in human nonpregnancy serum, rhesus serum incubated under similar conditions did not result in proteolysis of rhesus IGFBP-3, suggesting that the IGFBP-3 protease in human pregnancy serum is not present in the circulation of the rhesus monkey. To assess proteolytic activity in these sera, zymographic polyacrylamide gel analysis, using gelatin as a substrate, was performed. A minor band of proteolytic activity (72 kDa) was observed in all three species throughout gestation.(ABSTRACT TRUNCATED AT 400 WORDS) |
| تدمد: | 1945-7170 0013-7227 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad995aa649412b948f0eaf60e7afa1bc https://doi.org/10.1210/endo.132.4.7681762 |
| رقم الأكسشن: | edsair.doi.dedup.....ad995aa649412b948f0eaf60e7afa1bc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457170 00137227 |
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