Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry
| العنوان: | Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry |
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| المؤلفون: | Birgit Janicke, Megha Patel, Marek Feith, Zahra El-Schich, Kersti Alm |
| المصدر: | Applied Sciences Volume 10 Issue 3 Applied Sciences, Vol 10, Iss 3, p 750 (2020) |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Glycan, Cell- och molekylärbiologi, Motility, Cell morphology, lcsh:Technology, lcsh:Chemistry, 03 medical and health sciences, chemistry.chemical_compound, breast cancer, 0302 clinical medicine, Breast cancer, medicine, General Materials Science, lcsh:QH301-705.5, Instrumentation, 030304 developmental biology, Fluid Flow and Transfer Processes, 0303 health sciences, biology, lcsh:T, viability, Process Chemistry and Technology, General Engineering, medicine.disease, lcsh:QC1-999, Computer Science Applications, Sialic acid, lcsh:Biology (General), lcsh:QD1-999, motility, chemistry, lcsh:TA1-2040, sialic acid, Cell culture, 030220 oncology & carcinogenesis, Cancer cell, biology.protein, Cancer research, digital holographic cytometry, molecularly imprinted polymers, lcsh:Engineering (General). Civil engineering (General), Cytometry, lcsh:Physics, Cell and Molecular Biology |
| الوصف: | Breast cancer is the second most common cancer type worldwide and breast cancer metastasis accounts for the majority of breast cancer-related deaths. Tumour cells produce increased levels of sialic acid (SA) that terminates the monosaccharide on glycan chains of the glycosylated proteins. SA can contribute to cellular recognition, cancer invasiveness and increase the metastatic potential of cancer cells. SA-templated molecularly imprinted polymers (MIPs) have been proposed as promising reporters for specific targeting of cancer cells when deployed in nanoparticle format. The sialic acid-molecularly imprinted polymers (SA-MIPs), which use SA for the generation of binding sites through which the nanoparticles can target and stain breast cancer cells, opens new strategies for efficient diagnostic tools. This study aims at monitoring the effects of SA-MIPs on morphology and motility of the epithelial type MCF-7 and the highly metastatic MDAMB231 breast cancer cell lines, using digital holographic cytometry (DHC). DHC is a label-free technique that is used in cell morphology studies of e.g., cell volume, area and thickness as well as in motility studies. Here, we show that MCF-7 cells move slower than MDAMB231 cells. We also show that SA-MIPs have an effect on cell morphology, motility and viability of both cell lines. In conclusion, by using DH microscopy, we could detect SA-MIPs impact on different breast cancer cells regarding morphology and motility. |
| وصف الملف: | application/pdf |
| تدمد: | 2076-3417 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adb64ef9da7c30579f3c94d19d302179 https://doi.org/10.3390/app10030750 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....adb64ef9da7c30579f3c94d19d302179 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20763417 |
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