National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010 - 2012
| العنوان: | National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010 - 2012 |
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| المؤلفون: | Olga Perovic, Ranmini Kularatne, Jeanette Wadula, Adrienne Saif, G Elliott, Warren Lowman, Khine Swe Swe-Han, Ashika Singh-Moodley, Colleen Bamford, Andrew Whitelaw, Ruth Lekalakala, Trusha Nana, Else Marais, Adriano Duse, Melony Fortuin De-Smit |
| المساهمون: | Department of Clinical Laboratory Sciences, Faculty of Health Sciences |
| المصدر: | SAMJ: South African Medical Journal, Volume: 104, Issue: 8, Pages: 563-568, Published: AUG 2014 South African Medical Journal South African Medical Journal; Vol 104, No 8 (2014); 563-568 |
| بيانات النشر: | Health and Medical Publishing Group, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Cefotaxime, medicine.drug_class, Klebsiella pneumoniae, Cefepime, Antibiotics, Ceftazidime, Microbial Sensitivity Tests, Therapeutics, beta-Lactamases, Microbiology, South Africa, Antibiotic resistance, Ciprofloxacin, Drug Resistance, Bacterial, polycyclic compounds, Prevalence, Medicine, Humans, Piperacillin, Surveillance, biology, Extended-spectrum beta-lactamases, business.industry, General Medicine, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, bacterial infections and mycoses, Sentinel site, Carbapenems, Genes, bacteria, Nosocomial, business, Infection, Multiplex Polymerase Chain Reaction, Sentinel Surveillance, medicine.drug |
| الوصف: | BACKROUND: The increasing rates of antimicrobial resistance observed in the nosocomial pathogen Klebsiella pneumoniae are of major public health concern worldwide. OBJECTIVES: To describe the antibiotic susceptibility profiles of K. pneumoniae isolates from bacteraemic patients submitted by sentinel laboratories in five regions of South Africa from mid-2010 to mid-2012. Molecular methods were used to detect the most commonly found extended-spectrum beta-lactamase (ESBL) and carbapenemase resistance genes. METHODS: Thirteen academic centres serving the public healthcare sector in Gauteng, KwaZulu-Natal, Free State, Limpopo and Western Cape provinces submitted K. pneumoniae isolates from patients with bloodstream infections. Vitek 2 and MicroScan instruments were used for organism identification and susceptibility testing. Multiplex polymerase chain reactions (PCRs) were used to detect blaCTX-M, blaSHV and blaTEM genes in a proportion of the ESBL isolates. All isolates exhibiting reduced susceptibility to carbapenems were PCR tested for blaKPC and blaNDM-1 resistance genes. RESULTS: Overall, 68.3% of the 2 774 isolates were ESBL-positive, showing resistance to cefotaxime, ceftazidime and cefepime. Furthermore, 46.5% of all isolates were resistant to ciprofloxacin and 33.1% to piperacillin-tazobactam. The major ESBL genes were abundantly present in the sample analysed. Most isolates (95.5%) were susceptible to the carbapenems tested, and no isolates were positive for blaKPC or blaNDM1 There was a trend towards a decrease in susceptibility to most antibiotics. CONCLUSION: The high proportion of ESBL-producing K. pneumoniae isolates observed, and the prevalence of ESBL genes, are of great concern. Our findings represent a baseline for further surveillance in SA, and can be used for policy and treatment decisions. |
| وصف الملف: | text/html; application/pdf |
| اللغة: | English |
| تدمد: | 2078-5135 |
| DOI: | 10.7196/SAMJ.7617 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aea746190dced38f66560063545cfb23 http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0256-95742014000800025&lng=en&tlng=en |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....aea746190dced38f66560063545cfb23 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20785135 |
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| DOI: | 10.7196/SAMJ.7617 |