Mobile antibiotic resistome in wastewater treatment plants revealed by Nanopore metagenomic sequencing
| العنوان: | Mobile antibiotic resistome in wastewater treatment plants revealed by Nanopore metagenomic sequencing |
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| المؤلفون: | You Che, Yu Xia, Yu Yang, Lei Liu, Tong Zhang, An Dong Li |
| المصدر: | Microbiome, Vol 7, Iss 1, Pp 1-13 (2019) Microbiome |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Microbiology (medical), Gene Transfer, Horizontal, Streptogramins, Nanopore sequencing, Context (language use), Biology, Wastewater, Microbiology, lcsh:Microbial ecology, Conjugative plasmids, Mobile antibiotic resistome, 03 medical and health sciences, Nanopores, Plasmid, Microbial ecology, Bacterial Proteins, Host-tracking, Lincosamides, Genetic context, Illumina dye sequencing, Phylogeny, 030304 developmental biology, Genetics, 0303 health sciences, Bacteria, Sewage, 030306 microbiology, Research, High-Throughput Nucleotide Sequencing, Drug Resistance, Microbial, Sequence Analysis, DNA, Tetracycline, Resistome, Metagenomics, Horizontal gene transfer, lcsh:QR100-130, Macrolides |
| الوصف: | Background Wastewater treatment plants (WWTPs) are recognized as hotspots for horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs). Despite our understanding of the composition and distribution of ARGs in WWTPs, the genetic location, host, and fate of ARGs remain largely unknown. Results In this study, we combined Oxford Nanopore and Illumina metagenomics sequencing to comprehensively uncover the resistome context of influent, activated sludge, and effluent of three WWTPs and simultaneously track the hosts of the ARGs. The results showed that most of the ARGs detected in all compartments of the WWTPs were carried by plasmids. Transposons and integrons also showed higher prevalence on plasmids than on the ARG-carrying chromosome. Notably, integrative and conjugative elements (ICEs) carrying five types of ARGs were detected, and they may play an important role in facilitating the transfer of ARGs, particularly for tetracycline and macrolide-lincosamide-streptogramin (MLS). A broad spectrum of ARGs carried by plasmids (29 subtypes) and ICEs (4 subtypes) was persistent across the WWTPs. Host tracking showed a variety of antibiotic-resistant bacteria in the effluent, suggesting the high potential for their dissemination into receiving environments. Importantly, phenotype-genotype analysis confirmed the significant role of conjugative plasmids in facilitating the survival and persistence of multidrug-resistant bacteria in the WWTPs. At last, the consistency in the quantitative results for major ARGs types revealed by Nanopore and Illumina sequencing platforms demonstrated the feasibility of Nanopore sequencing for resistome quantification. Conclusion Overall, these findings substantially expand our current knowledge of resistome in WWTPs, and help establish a baseline analysis framework to study ARGs in the environment. Electronic supplementary material The online version of this article (10.1186/s40168-019-0663-0) contains supplementary material, which is available to authorized users. |
| اللغة: | English |
| تدمد: | 2049-2618 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aee842dadb907c34032e9616034f6a29 http://link.springer.com/article/10.1186/s40168-019-0663-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....aee842dadb907c34032e9616034f6a29 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20492618 |
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