Split-HaloTag imaging assay for sophisticated microscopy of protein–protein interactions in planta
| العنوان: | Split-HaloTag imaging assay for sophisticated microscopy of protein–protein interactions in planta |
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| المؤلفون: | Jutta Schulze, Henrik Buschmann, Rieke Minner-Meinen, Maria Behnecke, Cindy Tietge, Andreas Albrecht, Stefan Frank, Robert Hänsch, Rainer Matis, Peter Walla, Ralf R. Mendel, David Kaufholdt, Jan-Niklas Weber |
| المصدر: | Plant Communications |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | molybdenum cofactor biosynthesis complex, photostable fluorescent dyes, Plant Science, Biochemistry, Filamentous actin, Protein–protein interaction, Protein complex localization, Microscopy, Split-HaloTag imaging assay, Protein Interaction Domains and Motifs, Resource Article, Cytoskeleton, Molecular Biology, Chemistry, Botany, Polarization Microscopy, cytoskeleton, Cell Biology, Plants, Fluorescence, advanced microscopy, gateway cloning, protein–protein interaction, Covalent bond, Biophysics, Biotechnology |
| الوصف: | An ever-increasing number of intracellular multi-protein networks have been identified in plant cells. Split-GFP-based protein–protein interaction assays combine the advantages of in vivo interaction studies in a native environment with additional visualization of protein complex localization. Because of their simple protocols, they have become some of the most frequently used methods. However, standard fluorescent proteins present several drawbacks for sophisticated microscopy. With the HaloTag system, these drawbacks can be overcome, as this reporter forms covalent irreversible bonds with synthetic photostable fluorescent ligands. Dyes can be used in adjustable concentrations and are suitable for advanced microscopy methods. Therefore, we have established the Split-HaloTag imaging assay in plants, which is based on the reconstitution of a functional HaloTag protein upon protein–protein interaction and the subsequent covalent binding of an added fluorescent ligand. Its suitability and robustness were demonstrated using a well-characterized interaction as an example of protein–protein interaction at cellular structures: the anchoring of the molybdenum cofactor biosynthesis complex to filamentous actin. In addition, a specific interaction was visualized in a more distinctive manner with subdiffractional polarization microscopy, Airyscan, and structured illumination microscopy to provide examples of sophisticated imaging. Split-GFP and Split-HaloTag can complement one another, as Split-HaloTag represents an alternative option and an addition to the large toolbox of in vivo methods. Therefore, this promising new Split-HaloTag imaging assay provides a unique and sensitive approach for more detailed characterization of protein–protein interactions using specific microscopy techniques, such as 3D imaging, single-molecule tracking, and super-resolution microscopy. HaloTag is a self-labeling protein tag. The Split-HaloTag imaging assay that was developed in this study is based on the reconstitution of a functional HaloTag protein upon protein–protein interaction and the subsequent covalent binding of an added fluorescent ligand. This Split-HaloTag imaging assay provides a unique and sensitive approach for more detailed characterization of protein–protein interactions with sophisticated microscopy techniques. |
| اللغة: | English |
| تدمد: | 2590-3462 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aefb474b715a2fdd2379566899ef032e http://europepmc.org/articles/PMC8555439 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....aefb474b715a2fdd2379566899ef032e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25903462 |
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