Bimodular Peptide Synthetase SidE Produces Fumarylalanine in the Human Pathogen Aspergillus fumigatus
| العنوان: | Bimodular Peptide Synthetase SidE Produces Fumarylalanine in the Human Pathogen Aspergillus fumigatus |
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| المؤلفون: | Wieland Steinchen, Hubertus Haas, Markus Schrettl, Gerald Lackner, Hans-Martin Dahse, Sabiha Yasmin, Dirk Hoffmeister |
| المصدر: | Applied and Environmental Microbiology. 79:6670-6676 |
| بيانات النشر: | American Society for Microbiology, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Molecular Sequence Data, Virulence, Peptide, Human pathogen, Mycology, medicine.disease_cause, Applied Microbiology and Biotechnology, Aspergillus fumigatus, Microbiology, Plasmid, Fumarates, Escherichia coli, medicine, Peptide Synthases, Adenylylation, Phylogeny, chemistry.chemical_classification, Alanine, Base Sequence, Ecology, biology, Genetic Complementation Test, Blotting, Northern, biology.organism_classification, In vitro, chemistry, Sequence Alignment, Plasmids, Food Science, Biotechnology |
| الوصف: | The filamentous mold Aspergillus fumigatus causes invasive aspergillosis, a potentially life-threatening infectious disease, in humans. The sidE gene encodes a bimodular peptide synthetase and was shown previously to be strongly upregulated during initiation of murine lung infection. In this study, we characterized the two adenylation domains of SidE with the ATP-[ 32 P]pyrophosphate exchange assay in vitro , which identified fumarate and l -alanine, respectively, as the preferred substrates. Using full-length holo -SidE, fumarylalanine (FA) formation was observed in vitro . Furthermore, FA was identified in A. fumigatus culture supernatants under inducing conditions, unless sidE was genetically inactivated. As FA is structurally related to established pharmaceutical products exerting immunomodulatory activity, this work may contribute to our understanding of the virulence of A. fumigatus . |
| تدمد: | 1098-5336 0099-2240 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af20fae401da181c9cebd02904685889 https://doi.org/10.1128/aem.02642-13 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....af20fae401da181c9cebd02904685889 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985336 00992240 |
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