A monomer purified from Paris polyphylla (PP-22) triggers S and G2/M phase arrest and apoptosis in human tongue squamous cell carcinoma SCC-15 by activating the p38/cdc25/cdc2 and caspase 8/caspase 3 pathways
| العنوان: | A monomer purified from Paris polyphylla (PP-22) triggers S and G2/M phase arrest and apoptosis in human tongue squamous cell carcinoma SCC-15 by activating the p38/cdc25/cdc2 and caspase 8/caspase 3 pathways |
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| المؤلفون: | Jun-Yu Ke, Hui-Qun Huang, Rui-Song Gong, Wu Zhang, Wei-Dong Kong, Wan-Jing Cen, Ye-Rong Li, Jian-Wei Jiang |
| المصدر: | Tumor Biology. 37:14863-14872 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, MAPK/ERK pathway, Pyridines, Poly ADP ribose polymerase, Cyclin A, Apoptosis, Cell Cycle Proteins, Caspase 3, Biology, Caspase 8, p38 Mitogen-Activated Protein Kinases, 03 medical and health sciences, 0302 clinical medicine, Cell Line, Tumor, Cyclins, CDC2 Protein Kinase, Humans, cdc25 Phosphatases, Cyclin B1, Cell Proliferation, Cyclin-dependent kinase 1, Plant Extracts, Imidazoles, Nuclear Proteins, General Medicine, Protein-Tyrosine Kinases, Saponins, Antineoplastic Agents, Phytogenic, Molecular biology, Cyclin-Dependent Kinases, Tongue Neoplasms, DNA-Binding Proteins, G2 Phase Cell Cycle Checkpoints, 030104 developmental biology, Cyclin E2, 030220 oncology & carcinogenesis, S Phase Cell Cycle Checkpoints, Carcinoma, Squamous Cell, biology.protein, Cyclin A1, Poly(ADP-ribose) Polymerases, Tumor Suppressor Protein p53, Melanthiaceae, Transcription Factors |
| الوصف: | Recent studies have shown that the aqueous, ethanolic extracts and a monomer compound of Paris polyphylla exhibit anticancer activity toward several types of cancer cell lines, but the anticancer activity of (3β,17α,25R)-spirost-5-ene-3,17-diol 3-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranoside, a monomer isolated from P. polyphylla (PP), named PP-22, has not been reported previously. In this study, we investigated the effect of PP-22 on human tongue squamous cell carcinoma SCC-15 cells in vitro. MTT assays showed that PP-22 inhibited the growth of SCC-15 cells and had no obvious inhibitory effects on human liver L02 cells. Flow cytometry assays showed that the percentages of apoptotic cells were increased. In addition, cleaved caspase-8, cleaved caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP) could be detected by Western blotting. Flow cytometry also showed that PP-22 triggered S and G2/M phases arrest in SCC-15 cells, and on the other hand, the expression of cyclin A, cyclin E2, cyclin B1, phospho-cell division cycle2 (p-cdc2)(Tyr15), p-Wee1, Myt1, and p53 was upregulated. Moreover, p-p38 levels increased, p-extracellular signal-regulated kinase (ERK) levels decreased, and cdc25B expression was inhibited. Furthermore, the p38/mitogen-activated protein kinase (MAPK) inhibitor SB203580 reversed the increase of the expression level of p38, p-cdc2 (Tyr15), cleaved caspase 3, cleaved PARP, p-p53, and p53 and reversed the decrease in cdc25B expression. In conclusion, these results demonstrated that PP-22 activated p38, inhibited cdc25B, increased p-cdc2 (Tyr15), and triggered S and G2/M phase arrest, as well as activated p53 through the p38-p53 pathway, inhibited the MAPK/ERK pathway, activated the caspase 8/caspase 3 pathway, and triggered the extrinsic apoptotic pathway in SCC-15 cells. |
| تدمد: | 1423-0380 1010-4283 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af3edf6aa7217ab338b9dbae4b2e93a3 https://doi.org/10.1007/s13277-016-5376-4 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....af3edf6aa7217ab338b9dbae4b2e93a3 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14230380 10104283 |
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