Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry
| العنوان: | Simultaneous determination of nineteen major components in Qi She Pill by ultra-high-performance liquid chromatography–tandem mass spectrometry |
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| المؤلفون: | Li Qiufen, Yongjun Wang, Yan Zhao, Qiang Li, Zhong-liang Zhang, Ning Zhang, Simiao Du, Chunming Lv, Yongquan Zhou |
| المصدر: | Acta Pharmaceutica Sinica. B Acta Pharmaceutica Sinica B, Vol 4, Iss 5, Pp 384-393 (2014) |
| بيانات النشر: | Elsevier BV, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | 5-O-M, 5-O-methylvisammioside, Formic acid, Calibration curve, BER, berberine, PAL, palmatine, QSP, Qi She Pill, THPB, tetrahydroepiberberine, RA, rheumatoid arthritis, UHPLC–MS/MS, Mass spectrometry, CE, collision energy, ONO, ononin, chemistry.chemical_compound, Traditional Chinese medicine, Liquid chromatography–mass spectrometry, CSR, cervical spondylosis radiculopathy, TET, (+)-tetrandrine, Medicine, General Pharmacology, Toxicology and Pharmaceutics, AST-III, astragaloside III, THP, tetrahydropalmatine, SIN, sinomenine, CA, cholic acid, Chromatography, Traditional medicine, Active components, Quality assessment, business.industry, lcsh:RM1-950, SEA, senkyunolide A, FOR, formononetin, SEI, senkyunolide I, CCS, calycosin, GA, gallic acid, AST-IV, astragaloside IV, SRM, selective reaction monitoring, lcsh:Therapeutics. Pharmacology, Qi She Pill (QSP), chemistry, RA - Rheumatoid arthritis, Gradient elution, Original Article, CCSG, calycosin-7-O-β-d-glucoside, DAI, daidzein, TCM, traditional Chinese medicine, Ultra high performance, business, FAN, fangchinoline |
| الوصف: | Qi She Pill (QSP) is a traditional Chinese medicine (TCM) prescription that has been used in treating cervical spondylosis radiculopathy for many years. In this study, a simple and sensitive method using ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) on a reverse-phase C18 column was developed for the simultaneous determination of the 19 major components in QSP. We found that the optimum mobile phase for gradient elution was 0.1% formic acid and methanol. The correlation coefficients of all calibration curves were greater than 0.99. Recoveries measured at three concentration levels varied from 95.43% to 102.35%. Relative standard deviations of intra- and inter-day precisions were less than 4.45%. After successfully validating our method, we then applied it to the quantification of 19 components in QSP products to show that this method provides a new standard in quality assessment of TCM prescriptions containing multiple bioactive components. Graphical abstract An accurate and reliable UPLC–MS/MS method was developed to assess the multiple constituents in QSP. This method provides a new standard in quality control of QSP. Many components in the QSP prescription which are responsible for beneficial effects were detected for the first time. |
| تدمد: | 2211-3835 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afcffa21089bf03c23b2ae8226362f12 https://doi.org/10.1016/j.apsb.2014.05.003 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....afcffa21089bf03c23b2ae8226362f12 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22113835 |
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