Effectoromics-based identification of cell surface receptors in potato
| العنوان: | Effectoromics-based identification of cell surface receptors in potato |
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| المؤلفون: | Doret Wouters, Gerard Bijsterbosch, Xiao Lin, Vivianne G. A. A. Vleeshouwers, Carolina Aguilera-Galvez, Emmanouil Domazakis, Pieter J. Wolters |
| المصدر: | Plant Pattern Recognition Receptors. Humana Press Inc. Plant Pattern Recognition Receptors Methods in Molecular Biology ISBN: 9781493968589 |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Agroinfiltration, Protein infiltration, Apoplastic effector, Genetic mapping, Computational biology, Plant disease resistance, Proteomics, Solanum, 03 medical and health sciences, PBR Biodiversiteit en Genetische Variatie, Laboratorium voor Plantenveredeling, PVX agroinfection, Gene, Pattern recognition receptors (PRRs), biology, business.industry, Effector, Effectoromics, Yeast protein production, fungi, food and beverages, biology.organism_classification, Potato virus X, Biotechnology, Plant Breeding, 030104 developmental biology, Phytophthora infestans, EPS, business, PBR Biodiversity and genetic variation |
| الوصف: | In modern resistance breeding, effectors have emerged as tools for accelerating and improving the identification of immune receptors. Effector-assisted breeding was pioneered for identifying resistance genes (R genes) against Phytophthora infestans in potato (Solanum tuberosum). Here we show that effectoromics approaches are also well suitable for identifying pathogen recognition receptors (PRRs) that recognize apoplastic effectors. To detect genotypes that recognize apoplastic proteins of P. infestans, routine agroinfiltration and potato virus X (PVX) agroinfection methods can be applied. In addition, protein infiltrations are feasible for assessing responses to apoplastic effectors and aid in confirming results obtained from the aforementioned methods. Protocols for the effectoromics pipeline are provided, starting from phenotyping for effector responses, up to genotyping and PRR gene identification. |
| وصف الملف: | application/octet-stream; application/pdf |
| اللغة: | English |
| ردمك: | 978-1-4939-6858-9 978-1-4939-6859-6 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afd0b4039506428c96ccba5676ea9453 https://research.wur.nl/en/publications/effectoromics-based-identification-of-cell-surface-receptors-in-p |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....afd0b4039506428c96ccba5676ea9453 |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781493968589 9781493968596 |
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