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Magnetic Fluid Hyperthermia as Treatment Option for Pancreatic Cancer Cells and Pancreatic Cancer Organoids

التفاصيل البيبلوغرافية
العنوان: Magnetic Fluid Hyperthermia as Treatment Option for Pancreatic Cancer Cells and Pancreatic Cancer Organoids
المؤلفون: Ulf P. Neumann, Julian Palzer, Thomas Schmitz-Rode, Rianne D. W. Vaes, Benedikt Mues, Steven W.M. Olde Damink, Merel R. Aberle, Thorsten Cramer, Ioana Slabu, Richard Goerg, Anjali A. Roeth, Sander S. Rensen
المساهمون: Surgery, RS: NUTRIM - R2 - Liver and digestive health, MUMC+: MA Heelkunde (9)
المصدر: International Journal of Nanomedicine, 16, 2965-2981. Dove Medical Press Ltd
International Journal of Nanomedicine
سنة النشر: 2020
مصطلحات موضوعية: endocrine system diseases, Pancreatic Neoplasms/pathology, Cell, pancreatic cancer, Pharmaceutical Science, 02 engineering and technology, 01 natural sciences, International Journal of Nanomedicine, Drug Discovery, Original Research, Tumor, Adenocarcinoma/pathology, Cell Death, Chemistry, SPION, General Medicine, 021001 nanoscience & nanotechnology, Prognosis, magnetic fluid hyperthermia, Organoids, medicine.anatomical_structure, 0210 nano-technology, Carcinoma, Pancreatic Ductal/metabolism, Carcinoma, Pancreatic Ductal, magnetic nanoparticles, Cell Survival, Biophysics, Bioengineering, Pancreatic Ductal/metabolism, Adenocarcinoma, 010402 general chemistry, Cell Line, Biomaterials, In vivo, Pancreatic cancer, Cell Line, Tumor, medicine, Extracellular, Organoid, Humans, Hyperthermia, Viability assay, Clonogenic assay, Magnetic Phenomena, Carcinoma, Induced, Organic Chemistry, PDAC, Hyperthermia, Induced, medicine.disease, 0104 chemical sciences, Clone Cells, Pancreatic Neoplasms, Organoids/pathology, Cell culture, Cancer research
الوصف: Julian Palzer,1,2 Benedikt Mues,2 Richard Goerg,1,2 Merel Aberle,3 Sander S Rensen,3 Steven WM Olde Damink,1,3 Rianne DW Vaes,3 Thorsten Cramer,1,3 Thomas Schmitz-Rode,2 Ulf P Neumann,1,3 Ioana Slabu,2 Anjali A Roeth1,3 1Department of General, Visceral and Transplant Surgery, RWTH Aachen University Hospital, Aachen, Germany; 2Institute of Applied Medical Engineering, Helmholtz Institute Aachen, RWTH Aachen University Hospital, Aachen, Germany; 3Department of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, the NetherlandsCorrespondence: Anjali A Roeth Email aroeth@ukaachen.deIntroduction: Pancreatic ductal adenocarcinoma (PDAC) is a cancer with a meager prognosis due to its chemotherapy resistance. A new treatment method may be magnetic fluid hyperthermia (MFH). Magnetoliposomes (ML), consisting of superparamagnetic iron oxide nanoparticles (SPION) stabilized with a phospholipid-bilayer, are exposed to an alternating magnetic field (AMF) to generate heat. To optimize this therapy, we investigated the effects of MFH on human PDAC cell lines and 3D organoid cultures.Material and Methods: ML cytotoxicity was tested on Mia PaCa-2 and PANC-1 cells and on PDAC 3D organoid cultures, generated from resected tissue of patients. The MFH was achieved by AMF application with an amplitude of 40– 47 kA/m and a frequency of 270 kHz. The MFH effect on the cell viability of the cell lines and the organoid cultures was investigated at two different time points. Clonogenic assays evaluated the impairment of colony formation. Altering ML set-ups addressed differences arising from intra- vs extracellular ML locations.Results: Mia PaCa-2 and PANC-1 cells showed no cytotoxic effects at ML concentrations up to 300 μg(Fe)/mL and 225 μg(Fe)/mL, respectively. ML at a concentration of 225 μg(Fe)/mL were also non-toxic for PDAC organoid cultures. MFH treatment using exclusively extracellular ML presented the highest impact on cell viability. Clonogenic assays demonstrated remarkable impairment as long-term outcome in MFH-treated PDAC cell lines. Additionally, we successfully treated PDAC organoids with extracellular ML-derived MFH, resulting in notably reduced cell viabilities 2h and 24 h post treatment. Still, PDAC organoids seem to partly recover from MFH after 24 h as opposed to conventional 2D-cultures.Conclusion: Treatment with MFH strongly diminished pancreatic cancer cell viability in vitro, making it a promising treatment strategy. As organoids resemble the more advanced in vivo conditions better than conventional 2D cell lines, our organoid model holds great potential for further investigations.Keywords: magnetic nanoparticles, SPION, pancreatic cancer, PDAC, organoids, magnetic fluid hyperthermia
وصف الملف: text/html
تدمد: 1178-2013
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b03e3d4596432eea776d5d8f4a6d3976
https://pubmed.ncbi.nlm.nih.gov/33935496
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....b03e3d4596432eea776d5d8f4a6d3976
قاعدة البيانات: OpenAIRE
الوصف
تدمد:11782013