Generation and Propagation of MALDI Ion Packets Probed by Sheet-Like Nanosecond UV Laser Light
| العنوان: | Generation and Propagation of MALDI Ion Packets Probed by Sheet-Like Nanosecond UV Laser Light |
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| المؤلفون: | Kennosuke Hoshina, Tatsuro Shirota |
| المصدر: | Mass Spectrometry |
| بيانات النشر: | The Mass Spectrometry Society of Japan, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Materials science, proton transfer, Analytical chemistry, 010402 general chemistry, medicine.disease_cause, 01 natural sciences, Ion, law.invention, Matrix (chemical analysis), Time-of-Flight (TOF) mass spectrometry, law, Ionization, medicine, Irradiation, MALDI, Instrumentation, Spectroscopy, 010401 analytical chemistry, Photodissociation, photodissociation, Nanosecond, Laser, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, Original Article, CHCA, Ultraviolet |
| الوصف: | A sheet-like ultraviolet (UV) probe laser is used to investigate the ejection and propagation of ion packets of matrix CHCA, which are produced by matrix-assisted laser desorption and ionization (MALDI). Laser irradiation of the expanding MALDI plume induced photodissociation of the CHCA-related ions, which existed in a sheet-like volume, leading to their absence in their MALDI signal profiles. The MALDI spectra were measured under varying conditions: the temporal delay of the lasers and the distance of the sheet-like probe laser from the MALDI sample surface. It was found that the center of the (CHCA)H+ packets were ejected at 46±11 ns after MALDI laser irradiation, while the (CHCA)2H+ packets were ejected at 64±12 ns, regardless of the magnitude of acceleration static high-voltage in 3.5–5.5 kV. This suggests that (CHCA)2H+ is formed by a proton transfer reaction from (CHCA)H+ to (CHCA)2 in the heated condensed phase and/or near the surface. This study represents the first experimental determination of ion ejection time in the MALDI process, which is also applicable to other species in the MALDI plume. |
| تدمد: | 2186-5116 2187-137X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b04ca378ddf8d94747a0374fccc93fbd https://doi.org/10.5702/massspectrometry.a0071 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b04ca378ddf8d94747a0374fccc93fbd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21865116 2187137X |
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