Identification of lung cancer oncogenes based on the mRNA expression and single nucleotide polymorphism profile data
| العنوان: | Identification of lung cancer oncogenes based on the mRNA expression and single nucleotide polymorphism profile data |
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| المؤلفون: | Chen Y, Wen-Hsiung Li, Ai Yq, Xia Yx, Li Yf, Li Rq, Yibin Wang, Chang L, Mei Q |
| المصدر: | Neoplasma. 62(6) |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Orphan receptor, Cancer Research, Single-nucleotide polymorphism, Protein tyrosine phosphatase, Biology, medicine.disease, Molecular biology, PTPRM, Oncology, PFKP, medicine, Single Nucleotide Polymorphism Profile, Lung cancer, Gene |
| الوصف: | This study aimed to identify the oncogenes associated with lung cancer based on the mRNA and single nucleotide polymorphism (SNP) profile data. The mRNA expression profile data of GSE43458 (80 cancer and 30 normal samples) and SNP profile data of GSE33355 (61 pairs of lung cancer samples and control samples) were downloaded from Gene Expression Omnibus database. Common genes between the mRNA profile and SNP profile were identified as the lung cancer oncogenes. Risk subpathways of the selected oncogenes with the SNP locus were analyzed using the iSubpathwayMiner package in R. Moreover, protein-protein interaction (PPI) network of the oncogenes was constructed using the HPRD database and then visualized using the Cytoscape. Totally, 3004 DEGs (1105 up-regulated and 1899 down-regulated) and 125 significant SNPs closely related to 174 genes in the lung cancer samples were identified. Also, 39 common genes, like PFKP (phosphofructokinase, platelet) and DGKH-rs11616202 (diacylglycerol kinase, eta) that enriched in sub-pathways such as galactose metabolism, fructose and mannose metabolism, and pentose phosphate pathway, were identified as the lung cancer oncogenes. Besides, PIK3R1 (phosphoinositide-3-kinase, regulatory subunit 1), RORA (RAR-related orphan receptor A), MAGI3 (membrane associated guanylate kinase, WW and PDZ domain containing 3), PTPRM (protein tyrosine phosphatase, receptor type, M), and BMP6 (bone morphogenetic protein 6) were the hub genes in PPI network. Our study suggested that PFKP and DGKH that enriched in galactose metabolism, fructose and mannose metabolism pathway, as well as PIK3R1, RORA, and MAGI3, may be the lung cancer oncogenes. |
| تدمد: | 0028-2685 1161-6202 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b075cf83cc4c8a8b3da7e40b0be1ce80 https://pubmed.ncbi.nlm.nih.gov/26458310 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b075cf83cc4c8a8b3da7e40b0be1ce80 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00282685 11616202 |
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