Optimization of antiscorpion venom production
| العنوان: | Optimization of antiscorpion venom production |
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| المؤلفون: | S. Adiguzel, Özcan Özkan, Ayhan Filazi, C. Ates, I. Bozyigit |
| المصدر: | Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 12, Iss 3, Pp 390-399 (2006) Journal of Venomous Animals and Toxins including Tropical Diseases v.12 n.3 2006 The Journal of venomous animals and toxins including tropical diseases Universidade Estadual Paulista (UNESP) instacron:UNESP Scopus-Elsevier Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 12, Issue: 3, Pages: 390-399, Published: 2006 |
| بيانات النشر: | SciELO, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | lcsh:Arctic medicine. Tropical medicine, lcsh:RC955-962, Antivenom, Venom, Pharmacology, Toxicology, Median lethal dose, complex mixtures, scorpion, Pepsin, lcsh:RA1190-1270, lcsh:Zoology, Medicine, lcsh:QL1-991, ED50, lcsh:Toxicology. Poisons, antivenom, biology, business.industry, Androctonus crassicauda, Effective dose (pharmacology), Complete adjuvant, Infectious Diseases, Immunology, biology.protein, Animal Science and Zoology, Parasitology, business |
| الوصف: | The present study was carried out to produce highly efficient antivenom from a small number of telsons in a short time. Venom solution was prepared through maceration of telsons from Androctonus crassicauda (Olivier, 1807) collected in the Southeastern Anatolia Region, Turkey. Lethal dose 50% (LD50) of the venom solution injected into mice was 1 ml/kg (95% confidence interval; 0.8-1.3), according to probit analysis. Different adjuvants (Freund's Complete Adjuvant, Freund's Incomplete Adjuvant, and 0.4% aluminium phosphate), at increasing doses and combined with venom, were subcutaneously injected into horses on days 0, 14, 21, 28, 35, and 42 of the experiment. Antivenom was collected from the immunized horses on days 45, 48, and 51 using the pepsin digestive method. The antivenom effective dose 50% (ED50) in mice was 0.5 ml (95% confidence interval; 0.40-0.58), according to probit analysis. It was concluded that 0.5 ml antivenom neutralized a venom dose 35-fold higher than the venom LD50. Thus, highly potent antivenom could be produced from about 238 telsons in 51 days. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1678-9199 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b13e85597ae6c3de8aabee57fb0436f0 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300004 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b13e85597ae6c3de8aabee57fb0436f0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16789199 |
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