miR‐150‐5p suppresses the stem cell‐like characteristics of glioma cells by targeting the Wnt/β‐catenin signaling pathway
| العنوان: | miR‐150‐5p suppresses the stem cell‐like characteristics of glioma cells by targeting the Wnt/β‐catenin signaling pathway |
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| المؤلفون: | Jingchao Jiang, Weidong Tian, Weijie Zhu |
| المصدر: | Cell Biology International. 44:1156-1167 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Carcinogenesis, Biology, Stem cell marker, medicine.disease_cause, 03 medical and health sciences, 0302 clinical medicine, Cancer stem cell, Cell Line, Tumor, Glioma, medicine, Animals, Humans, Wnt Signaling Pathway, beta Catenin, Cell Proliferation, Mice, Inbred BALB C, Cell growth, Wnt signaling pathway, Cell Biology, General Medicine, Middle Aged, medicine.disease, Gene Expression Regulation, Neoplastic, MicroRNAs, 030104 developmental biology, 030220 oncology & carcinogenesis, Neoplastic Stem Cells, Cancer research, Catenin Beta-1, Female, Stem cell |
| الوصف: | Glioma is the most common brain tumor malignancy with high mortality and poor prognosis. Emerging evidence suggests that cancer stem cells are the key culprit in the development of cancer. MicroRNAs have been reported to be dysregulated in many cancers, while the mechanism underlying miR-150-5p in glioma progression and proportion of stem cells is unclear. The expression levels of miR-150-5p and catenin beta 1 (CTNNB1, which encodes β-catenin) were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. The expression levels of downstream genes of the Wnt/β-catenin pathway and stem cell markers were detected by qRT-PCR. Tumorigenesis was investigated by cell viability, colony formation, and tumor growth in vitro and in vivo. The interaction between miR-150-5p and β-catenin was explored via bioinformatics analysis and luciferase activity assay. We found that miR-150-5p was downregulated in glioma and its overexpression inhibited cell proliferation, colony formation, and tumor growth. Moreover, miR-150-5p directly suppressed CTNNB1 and negatively regulated the abundances of downstream genes of the Wnt/β-catenin pathway and stem cell markers. Furthermore, miR-150-5p expression was decreased and β-catenin level was enhanced in CD133+ glioma stem cells. Knockdown of miR-150-5p contributed to CD133- cells with stem cell-like phenotype, whereas overexpression of miR-150-5p suppressed CD133+ glioma stem cell-like characteristics. In conclusion, miR-150-5p inhibited the progression of glioma by controlling stem cell-like characteristics via regulating the Wnt/β-catenin pathway, providing a novel target for glioma treatment. |
| تدمد: | 1095-8355 1065-6995 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b17874719e7df3b9dd2297bec7f0aff8 https://doi.org/10.1002/cbin.11314 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....b17874719e7df3b9dd2297bec7f0aff8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10958355 10656995 |
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