Reduced number and function of peripheral dendritic cells in coeliac disease
| العنوان: | Reduced number and function of peripheral dendritic cells in coeliac disease |
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| المؤلفون: | B. Rovati, D. Piancatelli, G. Ricci, D. Millimaggi, S. Mazzocchi, M. Danova, Gino Roberto Corazza, Rachele Ciccocioppo, A. Cagnoni, I. Pesce |
| المصدر: | Clinical and experimental immunology 149 (2007): 487–496. info:cnr-pdr/source/autori:Ciccocioppo R, Ricci G, Rovati B, Pesce I, Mazzocchi S, Piancatelli D, Cagnoni A, Millimaggi D, Danova M, Corazza GR./titolo:Reduced number and function of peripheral dendritic cells in coeliac disease./doi:/rivista:Clinical and experimental immunology (Print)/anno:2007/pagina_da:487/pagina_a:496/intervallo_pagine:487–496/volume:149 |
| بيانات النشر: | Oxford University Press (OUP), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Myeloid, celiac, Translational Studies, Adolescent, Lymphocyte, Immunology, Antigen presentation, Cell Count, Human leukocyte antigen, Biology, Gliadin, Immunophenotyping, Pathogenesis, Aged, Celiac Disease, Cell Differentiation, Cytokines, Dendritic Cells, Female, Flow Cytometry, Humans, Lymphocyte Culture Test, Mixed, Middle Aged, Immune system, Internal medicine, medicine, Immunology and Allergy, Lymphocyte Culture Test, Antigen-presenting cell, Dendritic cell, Mixed, Endocrinology, medicine.anatomical_structure |
| الوصف: | Summary Dendritic cells (DC) play a pivotal role in shaping the immune response in both physiological and pathological conditions. In peripheral blood at least two subsets, the myeloid and plasmacytoid, have been described as having different T stimulatory functions and a variable degree of maturation. Certainly, antigen presentation plays a crucial role in the pathogenesis of coeliac disease and circulating immune cells are thought to reflect the state of immune response within the gut. Therefore, we aimed to investigate the quantitative and phenotypical modifications of peripheral blood DC, together with their functional properties, in this pathological condition. Blood samples from 11 untreated patients before and after a course of gluten-free diet, 27 treated patients and 14 controls underwent flow-cytometric analysis, while immunomagnetically sorted DC from the CD patients and eight human leucocyte antigen (HLA)-DQ2/8+ bone marrow donors were used to evaluate maturation status through the CD83 expression, cytokine profile for interleukin (IL)-6, IL-10, IL-12 and interferon (IFN)-α by enzyme-linked immunosorbent assay (ELISA), and functional properties by mixed leucocyte reaction before and after pulsing with digested gliadin. We found that in both untreated and treated patients, a significant reduction of the entire DC population, mainly the plasmacytoid subset, in comparison to healthy controls was observed. In active disease, an impaired allogenic lymphocyte reaction and a significant reduction of IFN-α production, paralleled by the presence of a more immature status, were also demonstrated. All the latter modifications have been reverted by pulsing DC with digested gliadin. |
| تدمد: | 1365-2249 0009-9104 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b19ecfa28226a16ed7cc13cf0e19954e https://doi.org/10.1111/j.1365-2249.2007.03431.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b19ecfa28226a16ed7cc13cf0e19954e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652249 00099104 |
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