Functional and molecular immune response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes against pathogen-associated molecular patterns and bacteria
| العنوان: | Functional and molecular immune response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes against pathogen-associated molecular patterns and bacteria |
|---|---|
| المؤلفون: | M.M. Costa, María Prado-Álvarez, Philippe Roch, Beatriz Novoa, Camino Gestal, Antonio Figueras, Hui Li |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Elsevier BV, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Vibrio anguillarum, PAMPs, Hemocytes, Gram-negative bacteria, Aquatic Science, Nitric Oxide, NO, Micrococcus, Microbiology, chemistry.chemical_compound, Phagocytosis, Animals, Environmental Chemistry, Respiratory Burst, Vibrio, Mytilus, biology, Gene Expression Profiling, Pathogen-associated molecular pattern, Mytilin, Zymosan, AMPs, ROS, General Medicine, biology.organism_classification, Myticin, Respiratory burst, Mytilus galloprovincialis, Gene Expression Regulation, chemistry, Mussel, Lysozyme |
| الوصف: | 9 páginas, 6 figuras, 1 tabla The effect of live bacteria (Micrococcus lysodeikticus and Vibrio anguillarum), and PAMPs (poly I:C, zymosan, LPS, LTA and CpG) on the production of intermediate toxic radicals (respiratory burst activity and production of nitric oxide) and mytilin B, myticin C and lysozyme gene expression was studied in vivo and in vitro. In vitro, bacteria were able to modulate the haemocytes’ respiratory burst activity, being significantly increased after 6 h of incubation. The effect of pathogen-associated molecular patterns (PAMPs) was also studied. Zymosan produced an increase of the PMA-mediated response but an inhibition of the zymosan-mediated response. A significant increase of nitric oxide production was found at all the sampled time points (1, 3 and 6 h) in comparison with controls on both, the Gram-positive and Gram-negative bacteria. The in vivo responses measured on haemocytes after M. lysodeikticus injection were faster than those induced by V. anguillarum. However, V. anguillarum induced stronger in vitro effects. Mytilin B, myticin C and lysozyme in vitro gene expression, occurred at short times after infection. The maximum in vitro expression was detected 3 h post-infection. The differences between M. lysodeikticus and V. anguillarum in different measured parameters may suggest that different signalling pathways might be involved. Moreover, among all assayed PAMPs, LPS elicited the highest response. This research has been supported by the Ministerio de Ciencia e Innovación (project AGL2003-02454) and by European Community (project IMAQUANIM (FOOD-CT-2005-007103)). |
| تدمد: | 1050-4648 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1b8533dba10a8e790a67b8b1b6fe67e https://doi.org/10.1016/j.fsi.2009.02.001 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b1b8533dba10a8e790a67b8b1b6fe67e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10504648 |
|---|