Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (Sillago sihama)
| العنوان: | Comparative Transcriptome Analysis of Gill Tissue in Response to Hypoxia in Silver Sillago (Sillago sihama) |
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| المؤلفون: | Xinghua Lin, Fei-Xiang He, Jiawang Yu, Hongjuan Shi, Yang Huang, Yulei Zhang, Guangli Li, Wanida Saetan, Changxu Tian |
| المصدر: | Animals : an Open Access Journal from MDPI Animals Volume 10 Issue 4 Animals, Vol 10, Iss 628, p 628 (2020) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | hypoxia stress response, Sillago sihama, Steroid biosynthesis, Article, Transcriptome, chemistry.chemical_compound, lcsh:Zoology, Gene expression, lcsh:QL1-991, RNA-Seq, KEGG, lcsh:Veterinary medicine, General Veterinary, biology, Cytochrome P450, Metabolism, Glutathione, biology.organism_classification, Molecular biology, chemistry, biology.protein, gene expression, lcsh:SF600-1100, Animal Science and Zoology |
| الوصف: | Silver sillago (Sillago sihama) is a commercially important marine fish species in East Asia. In this study, we compared the transcriptome response to hypoxia stress in the gill tissue of S. sihama. The fish were divided into four groups, such as 1 h of hypoxia (hypoxia1h, DO = 1.5 ± 0.1 mg/L), 4 h of hypoxia (hypoxia4h, DO = 1.5 ± 0.1 mg/L), 4 h of reoxygen (reoxygen4h, DO = 8.0 ± 0.2 mg/L) after 4 h of hypoxia (DO = 1.5 mg/L), and normoxia or control (DO = 8.0 ± 0.2 mg/L) groups. Compared to the normoxia group, a total of 3550 genes were identified as differentially expressed genes (DEGs) (log2foldchange > 1 and padj < 0.05), including 1103, 1451 and 996 genes in hypoxia1h, hypoxia4h and reoxygen4h groups, respectively. Only 247 DEGs were differentially co-expressed in all treatment groups. According to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, DEGs were significantly enriched in steroid biosynthesis, biosynthesis of amino acids, glutathione metabolism and metabolism of xenobiotics by cytochrome P450, ferroptosis and drug metabolism&mdash cytochrome P450 pathways. Of these, the cytochrome P450 (CYP) and glutathione S-transferase (GST) gene families were widely expressed. Our study represents the insights into the underlying molecular mechanisms of hypoxia stress. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-2615 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1d3d0eaa9bfdaac312bcec8e3f74b84 http://europepmc.org/articles/PMC7222756 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b1d3d0eaa9bfdaac312bcec8e3f74b84 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762615 |
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