GSK-3β activation is required for ZIP-induced disruption of learned fear
| العنوان: | GSK-3β activation is required for ZIP-induced disruption of learned fear |
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| المؤلفون: | Jeongyeon Kim, Sukwoon Song, Sukwoo Choi, Beomjong Song, Kyungjoon Park, Sukwon Lee, Ingie Hong, Sewon Park, Ji Hye Kim, Junghwa Lee |
| المصدر: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) |
| بيانات النشر: | Nature Publishing Group UK, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Physiology, lcsh:Medicine, macromolecular substances, Cell-Penetrating Peptides, Article, Small hairpin RNA, Rats, Sprague-Dawley, 03 medical and health sciences, Memory erasure, Lipopeptides, 0302 clinical medicine, Memory, medicine, Animals, Learning, Phosphorylation, lcsh:Science, GSK3B, Protein Kinase C, Gene knockdown, Multidisciplinary, Glycogen Synthase Kinase 3 beta, Chemistry, Kinase, lcsh:R, Long-term potentiation, Fear, Cell biology, Rats, 030104 developmental biology, Mechanism of action, Models, Animal, lcsh:Q, medicine.symptom, Depotentiation, 030217 neurology & neurosurgery, Neuroscience, Signal Transduction |
| الوصف: | The myristoylated zeta inhibitory peptide (ZIP), which was originally developed as a protein kinase C/Mζ (PKCζ/PKMζ) inhibitor, is known to produce the loss of different forms of memories. However, ZIP induces memory loss even in the absence of PKMζ, and its mechanism of action, therefore, remains elusive. Here, through a kinome-wide screen, we found that glycogen synthase kinase 3 beta (GSK-3β) was robustly activated by ZIP in vitro. ZIP induced depotentiation (a cellular substrate of memory erasure) of conditioning-induced potentiation at LA synapses, and the ZIP-induced depotentiation was prevented by a GSK-3β inhibitor, 6-bromoindirubin-3-acetoxime (BIO-acetoxime). Consistently, GSK-3β inhibition by BIO-acetoxime infusion or GSK-3β knockdown by GSK-3β shRNA in the LA attenuated ZIP-induced disruption of learned fear. Furthermore, conditioned fear was decreased by expression of a non-inhibitable form of GSK-3β in the LA. Our findings suggest that GSK-3β activation is a critical step for ZIP-induced disruption of memory. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b349dde54c550d88368edd2771e99a16 http://europepmc.org/articles/PMC7588416 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b349dde54c550d88368edd2771e99a16 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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