Autoantibodies that stabilize the molecular interaction of Ku antigen with DNA-dependent protein kinase catalytic subunit
| العنوان: | Autoantibodies that stabilize the molecular interaction of Ku antigen with DNA-dependent protein kinase catalytic subunit |
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| المؤلفون: | Westley H. Reeves, J J Langdon, Carter Th, Ogasawara T, Mary Anne Dooley, A. K. Ajmani, Minoru Satoh, John B. Winfield, Lovorka Stojanov, Hanno B. Richards, Jianbo Wang |
| المصدر: | Clinical and Experimental Immunology. 105:460-467 |
| بيانات النشر: | Oxford University Press (OUP), 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Chemical Phenomena, Protein subunit, Immunoblotting, Immunology, DNA-Activated Protein Kinase, Protein Serine-Threonine Kinases, Biology, Autoantigens, Catalysis, Epitope, Autoimmune Diseases, DNA-Dependent Protein Kinase Catalytic Subunit, Antigen-Antibody Reactions, Histones, Antigen, Humans, Immunology and Allergy, Ku Autoantigen, DNA-PKcs, Autoantibodies, Chemistry, Physical, Antigen processing, Immune Sera, DNA Helicases, Autoantibody, Nuclear Proteins, Antigens, Nuclear, Original Articles, DNA, Precipitin Tests, Molecular biology, Immune complex, DNA-Binding Proteins, enzymes and coenzymes (carbohydrates), biological phenomena, cell phenomena, and immunity |
| الوصف: | DNA-dependent protein kinase (DNA-PK) consists of a DNA binding subunit (Ku autoantigen), and a catalytic subunit (DNA-PKcs). In the present study, human autoantibodies that recognize novel antigenic determinants of DNA-PK were identified. One type of autoantibody stabilized the interaction of DNA-PKcs with Ku and recognized the DNA-PKcs–Ku complex, but not biochemically purified DNA-PKcs. Another type recognized purified DNA-PKcs. Autoantibodies to Ku (p70/p80 heterodimer), ‘stabilizing’ antibodies, and antibodies to DNA-PKcs comprise a linked autoantibody set, since antibodies recognizing purified DNA-PKcs were strongly associated with stabilizing antibodies, whereas stabilizing antibodies were strongly associated with anti-Ku. This hierarchical pattern of autoantibodies specific for components of DNA-PK (anti-Ku>stabilizing antibodies>anti-DNA-PKcs) may have implications for the pathogenesis of autoimmunity to DNA-PK and other chromatin particles. The data raise the possibility that altered antigen processing and/or stabilization of the DNA-PKcs–Ku complex due to autoantibody binding could play a role in spreading autoimmunity from Ku to the weakly associated antigen DNA-PKcs. |
| تدمد: | 1365-2249 0009-9104 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3a344a96e88aa6e051a783aebca58da https://doi.org/10.1046/j.1365-2249.1996.d01-775.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b3a344a96e88aa6e051a783aebca58da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652249 00099104 |
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