Cigarette smoke induces overexpression of active human cathepsin S in lungs from current smokers with or without COPD
| العنوان: | Cigarette smoke induces overexpression of active human cathepsin S in lungs from current smokers with or without COPD |
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| المؤلفون: | Thomas Baranek, Agnès Petit-Courty, Andrea C. Schamberger, Fabien Lecaille, Ahlame Saidi, Pierre-Marie Andrault, Oliver Eickelberg, Thibault Chazeirat, Damien Sizaret, Mylène Wartenberg, Serge Guyétant, Elisabeth Hennen, Gilles Lalmanach, Justine Renault, Yves Courty, Claudia A. Staab-Weijnitz |
| المساهمون: | Université de Tours (UT), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Ludwig-Maximilians-Universität München (LMU), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), brea, deborah, Centre d'Etude des Pathologies Respiratoires (CEPR), UMR 1100. Equipe 2 'Mécanismes Protéolytiques dans l'Inflammation' (CEPR. Equipe 2), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Comprehensive Pneumology Center - Institute of Lung Biology and Disease (iLBD), German Center for Lung Research, Anatomie et cytologie pathologique [Tours], Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), INSERM, Université de Tours, Région Centre-Val de Loire (project BPCO-LYSE no. 2015103986), Deutsche Forschungsgemeinschaft (DFG) within the Research Training Group DFG GRK2338, Helmholtz Zentrum München = German Research Center for Environmental Health, Lalmanach, Gilles, Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Gonzalez, Loïc |
| المصدر: | American Journal of Physiology-Lung Cellular and Molecular Physiology American Journal of Physiology-Lung Cellular and Molecular Physiology, American Physiological Society, 2019, 317 (5), pp.L625-L638. ⟨10.1152/ajplung.00061.2019⟩ Am. J. Physiol. Lung Cell Mol. Physiol. 317, L625-L638 (2019) |
| بيانات النشر: | HAL CCSD, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Pulmonary and Respiratory Medicine, Male, Physiology, oxidation, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Respiratory Mucosa, smokers, medicine.disease_cause, Cigarette Smoking, 03 medical and health sciences, Pulmonary Disease, Chronic Obstructive, 0302 clinical medicine, Cigarette smoking, Physiology (medical), Cigarette smoke, Medicine, Humans, COPD, cysteine protease, Cigarette Smoke, Copd, Cysteine Protease, Oxidation, Smokers, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Cells, Cultured, Cathepsin S, Aged, Protease, business.industry, cigarette smoke, Epithelial Cells, Cell Biology, Middle Aged, medicine.disease, Cysteine protease, Cathepsins, 3. Good health, respiratory tract diseases, [SDV] Life Sciences [q-bio], Oxidative Stress, 030104 developmental biology, 030228 respiratory system, Case-Control Studies, Immunology, Female, business, Lung tissue, Oxidative stress |
| الوصف: | International audience; Cigarette smoking has marked effects on lung tissue, including induction of oxidative stress, inflammatory cell recruitment, and a protease/antiprotease imbalance. These effects contribute to tissue remodeling and destruction resulting in loss of lung function in chronic obstructive pulmonary disease (COPD) patients. Cathepsin S (CatS) is a cysteine protease that is involved in the remodeling/degradation of connective tissue and basement membrane. Aberrant expression or activity of CatS has been implicated in a variety of diseases, including arthritis, cancer, cardiovascular, and lung diseases. However, little is known about the effect of cigarette smoking on both CatS expression and activity, as well as its role in smoking-related lung diseases. Here, we evaluated the expression and activity of human CatS in lung tissues from never-smokers and smokers with or without COPD. Despite the presence of an oxidizing environment, CatS expression and activity were significantly higher in current smokers (both non-COPD and COPD) compared with never-smokers, and correlated positively with smoking history. Moreover, we found that the exposure of primary human bronchial epithelial cells to cigarette smoke extract triggered the activation of P2X7 receptors, which in turns drives CatS upregulation. The present data suggest that excessive CatS expression and activity contribute, beside other proteases, to the deleterious effects of cigarette smoke on pulmonary homeostasis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1040-0605 1522-1504 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3ccbff970691c72a8be55cbc9f06d21 https://hal.archives-ouvertes.fr/hal-03604585 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b3ccbff970691c72a8be55cbc9f06d21 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10400605 15221504 |
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