أعرض في EDS

Circulating miRNA repertoire as a biomarker of metabolic and reproductive states in rainbow trout

التفاصيل البيبلوغرافية
العنوان: Circulating miRNA repertoire as a biomarker of metabolic and reproductive states in rainbow trout
المؤلفون: Cervin Guyomar, Emilie Cardona, Julien Bobe, Jérôme Montfort, John H. Postlethwait, Samia Guendouz, Thomas Desvignes, Sandrine Skiba-Cassy
المساهمون: Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute of Neurosciences, Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-BioCampus (BCM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), NSF USA grant PLR-1543383, NIH USA grant R01OD011116, France Génomique National infrastructure, ANR-16-CE20-0001,EggPreserve,Iddentification de protéines du fluide ovarien impliquées dans la préservation de la qualité des oeufs de poisson(2016), European Project: PFEA470016FA1000002,NutriEgg, Avril, Pascale, Iddentification de protéines du fluide ovarien impliquées dans la préservation de la qualité des oeufs de poisson - - EggPreserve2016 - ANR-16-CE20-0001 - AAPG2016 - VALID, European Maritime and Fisheries Fund - NutriEgg - PFEA470016FA1000002 - INCOMING, Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-École nationale supérieure agronomique de Toulouse (ENSAT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)
المصدر: BMC Biology
BMC Biology, 2021, 19 (1), ⟨10.1186/s12915-021-01163-5⟩
BMC Biology, Vol 19, Iss 1, Pp 1-19 (2021)
BMC Biology, BioMed Central, 2021, 19 (1), ⟨10.1186/s12915-021-01163-5⟩
بيانات النشر: HAL CCSD, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Circulating mirnas, [SDV.BA] Life Sciences [q-bio]/Animal biology, Physiology, QH301-705.5, media_common.quotation_subject, Plant Science, The authors Emilie Cardona and Cervin Guyomar contributed equally to this work, Biology, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Structural Biology, Blood plasma, microRNA, mir375, Animals, Humans, Biology (General), Ovulation, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, media_common, Genetics, 0303 health sciences, Reproductive success, Repertoire, Reproduction, [SDV.BA]Life Sciences [q-bio]/Animal biology, myomiR, Cell Biology, Biomarker, MicroRNAs, Fish, mir202, Biological fluid, 030220 oncology & carcinogenesis, Oncorhynchus mykiss, Biomarker (medicine), Rainbow trout, Female, Non-invasive phenotyping, General Agricultural and Biological Sciences, Biomarkers, Developmental Biology, Biotechnology, Research Article
الوصف: Background Circulating miRNAs (c-miRNAs) are found in most, if not all, biological fluids and are becoming well-established non-invasive biomarkers of many human pathologies. However, their features in non-pathological contexts and whether their expression profiles reflect normal life history events have received little attention, especially in non-mammalian species. The aim of the present study was to investigate the potential of c-miRNAs to serve as biomarkers of reproductive and metabolic states in fish. Results The blood plasma was sampled throughout the reproductive cycle of female rainbow trout subjected to two different feeding regimes that triggered contrasting metabolic states. In addition, ovarian fluid was sampled at ovulation, and all samples were subjected to small RNA-seq analysis, leading to the establishment of a comprehensive miRNA repertoire (i.e., miRNAome) and enabling subsequent comparative analyses to a panel of RNA-seq libraries from a wide variety of tissues and organs. We showed that biological fluid miRNAomes are complex and encompass a high proportion of the overall rainbow trout miRNAome. While sharing a high proportion of common miRNAs, the blood plasma and ovarian fluid miRNAomes exhibited strong fluid-specific signatures. We further revealed that the blood plasma miRNAome significantly changed depending on metabolic and reproductive states. We subsequently identified three evolutionarily conserved muscle-specific miRNAs or myomiRs (miR-1-1/2-3p, miR-133a-1/2-3p, and miR-206-3p) that accumulated in the blood plasma in response to high feeding rates, making these myomiRs strong candidate biomarkers of active myogenesis. We also identified miR-202-5p as a candidate biomarker for reproductive success that could be used to predict ovulation and/or egg quality. Conclusions Together, these promising results reveal the high potential of c-miRNAs, including evolutionarily conserved myomiRs, as physiologically relevant biomarker candidates and pave the way for the use of c-miRNAs for non-invasive phenotyping in various fish species.
وصف الملف: application/pdf
اللغة: English
تدمد: 1741-7007
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3f4f47dd802f1106192eebbd6d0ecd6
https://hal.inrae.fr/hal-03436712/file/2021_Cardona_BMCBiology.pdf
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....b3f4f47dd802f1106192eebbd6d0ecd6
قاعدة البيانات: OpenAIRE
الوصف
تدمد:17417007