Phospholipase D2 in prostate cancer: protein expression changes with Gleason score
| العنوان: | Phospholipase D2 in prostate cancer: protein expression changes with Gleason score |
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| المؤلفون: | Norman J. Maitland, Martin G. Rumsby, Daniel M. Berney, Sylvain G. Bourgoin, Karen Hogg, Amanda R. Noble, Rakesh Suman |
| المصدر: | British Journal of Cancer |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Cancer Research, Stromal cell, Carcinogenesis, Cell Survival, Biology, urologic and male genital diseases, medicine.disease_cause, Article, Cell growth, 03 medical and health sciences, Prostate cancer, 0302 clinical medicine, Prostate, Neoplasms, Phospholipase D, medicine, Humans, Viability assay, Cell Proliferation, 030304 developmental biology, 0303 health sciences, Time-lapse imaging, Chromogranin A, Cancer, medicine.disease, Gene Expression Regulation, Neoplastic, Prostatic Neoplasms, Castration-Resistant, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Disease Progression, Cancer research, biology.protein, Neoplasm Grading, Signal Transduction |
| الوصف: | BackgroundPhospholipases D1 and D2 (PLD1/2) are implicated in tumorigenesis through their generation of the signalling lipid phosphatidic acid and its downstream effects. Inhibition of PLD1 blocks prostate cell growth and colony formation. Here a role for PLD2 in prostate cancer (PCa), the major cancer of men in the western world, is examined.MethodsPLD2 expression was analysed by immunohistochemistry and western blotting. The effects of PLD2 inhibition on PCa cell viability and cell motility were measured using MTS, colony forming and wound-healing assays.ResultsPLD2 protein is expressed about equally in luminal and basal prostate epithelial cells. In cells from different Gleason-scored PCa tissue PLD2 protein expression is generally higher than in non-tumorigenic cells and increases in PCa tissue scored Gleason 6–8. PLD2 protein is detected in the cytosol and nucleus and had a punctate appearance. In BPH tissue stromal cells as well as basal and luminal cells express PLD2. PLD2 protein co-expresses with chromogranin A in castrate-resistant PCa tissue. PLD2 inhibition reduces PCa cell viability, colony forming ability and directional cell movement.ConclusionsPLD2 expression correlates with increasing Gleason score to GS8. PLD2 inhibition has the potential to reduce PCa progression. |
| تدمد: | 1532-1827 0007-0920 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b43a8e2baf4a13c2060993c556df52b2 https://doi.org/10.1038/s41416-019-0610-7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b43a8e2baf4a13c2060993c556df52b2 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15321827 00070920 |
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