Serum sCD14, PGLYRP2 and FGA as potential biomarkers for multidrug‐resistant tuberculosis based on data‐independent acquisition and targeted proteomics
| العنوان: | Serum sCD14, PGLYRP2 and FGA as potential biomarkers for multidrug‐resistant tuberculosis based on data‐independent acquisition and targeted proteomics |
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| المؤلفون: | Ting-Ting Jiang, Li-Ying Shi, Ji-Cheng Li, Li-Liang Wei, Jing Chen, Zhi-Bin Li, Yi Yu, Wen-Jing Yi, Huai Huang, Yu-Shuai Han |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, Proteomics, Quality Control, 0301 basic medicine, data‐independent acquisition, PGLYRP2, Tuberculosis, Lipopolysaccharide Receptors, Mass Spectrometry, 03 medical and health sciences, 0302 clinical medicine, FGA, Bacterial Proteins, Tuberculosis, Multidrug-Resistant, medicine, Humans, Data-independent acquisition, Protein Interaction Maps, Principal Component Analysis, business.industry, Isoniazid, Area under the curve, Fibrinogen, Original Articles, Cell Biology, sCD14, medicine.disease, Blood proteins, Multiple drug resistance, parallel reaction monitoring, Gene Ontology, 030104 developmental biology, ROC Curve, 030220 oncology & carcinogenesis, Immunology, biomarker, Molecular Medicine, Biomarker (medicine), Original Article, Female, Carrier Proteins, business, multidrug‐resistant tuberculosis, Biomarkers, Rifampicin, medicine.drug |
| الوصف: | Multidrug‐resistant tuberculosis (MDR‐TB), defined as tuberculosis (TB) resistant to at least isoniazid and rifampicin, is a major concern of TB control worldwide. However, the diagnosis of MDR‐TB remains a huge challenge to its prevention and control. To identify new diagnostic methods for MDR‐TB, a mass spectrometry strategy of data‐independent acquisition and parallel reaction monitoring was used to detect and validate differential serum proteins. The bioinformatic analysis showed that the functions of differential serum proteins between the MDR‐TB group and the drug‐sensitive tuberculosis group were significantly correlated to the complement coagulation cascade, surface adhesion and extracellular matrix receptor interaction, suggesting a disorder of coagulation in TB. Here, we identified three potential candidate biomarkers such as sCD14, PGLYRP2 and FGA, and established a diagnostic model using these three candidate biomarkers with a sensitivity of 81.2%, a specificity of 90% and the area under the curve value of 0.934 in receiver operation characteristics curve to diagnose MDR‐TB. Our study has paved the way for a novel method to diagnose MDR‐TB and may contribute to elucidate the mechanisms underlying MDR‐TB. |
| تدمد: | 1582-4934 1582-1838 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b45f4c366d6afe19eab12e318a2f4335 https://doi.org/10.1111/jcmm.15796 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b45f4c366d6afe19eab12e318a2f4335 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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