Extraction of Datura metel L. leaves with ethanol as a solvent gave a group of steroids, including two unique 1,10-seco-withanolides (1, 4), an unusual nitrogen-containing withanolides (2), one undescribed saponin (3), two withanolides with a carbohydrate (5, 6), and one C21 steroid (7). These compounds' structures were identified based on HR-ESI-MS and 1H, 13C NMR data analyses, also compared with data from the document. Some compounds showed moderate inhibition on NO production in lipopolysaccharide-stimulated RAW 264.7 cells.
