Real-time polymerase chain reaction for detection and enumeration of Staphylococcus aureus and Streptococccus agalactiae using different milk samplings
| العنوان: | Real-time polymerase chain reaction for detection and enumeration of Staphylococcus aureus and Streptococccus agalactiae using different milk samplings |
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| المؤلفون: | Aline Gerato Dibbern, Juliano Leonel Gonçalves, Bruna Gomes Alves, Juliana Regina Barreiro, Kevin Anderson, Marcos Veiga dos Santos |
| المصدر: | Revista Brasileira de Zootecnia, Volume: 52, Article number: e20210086, Published: 07 APR 2023 |
| بيانات النشر: | Revista Brasileira de Zootecnia, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | qPCR, molecular microbiology, identification, Animal Science and Zoology, bovine mastitis |
| الوصف: | The objective of the present study was to evaluate the qPCR for detection and enumeration of Staphylococcus aureus and Streptococcus agalactiae using different milk samplings in comparison to the conventional microbiology. Four dairy herds with a history of subclinical mastitis caused by S. aureus and S. agalactiae were selected. Sampling approach included milk samples from bulk tank (BT), cow level (composite samples, CO), and mammary quarter level (MQ) from 785 lactating cows. Three consecutive monthly milk samplings were carried out, totaling 3347 MQ milk samples, 912 CO, and 12 from BT. All collected milk samples were subjected to conventional microbiology and qPCR for detection and enumeration of S. aureus and S. agalactiae. The qPCR showed 71.5% of diagnostic sensitivity for S. aureus isolated from MQ milk samples, 71.8% for CO, and 50% for BT milk samples compared with conventional microbiology methodology. Taken together, the diagnostic sensitivity for S. agalactiae isolated from MQ milk samples was 90.2, 87.7 for CO, and 90.9% for BT milk samples. In general, the qPCR methodology enabled the detection of S. aureus and S. agalactiae, regardless of the type of milk sampling. The direct use of milk samples to estimate the counting of S. aureus by qPCR demonstrated lower sensitivity than the counting of S. agalactiae, which can be explained by the pathogen infection dynamics and differences in milk sample type. |
| وصف الملف: | text/html |
| تدمد: | 1806-9290 1516-3598 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4ad45192769bbb99e22e58110c9dd8f https://doi.org/10.37496/rbz5220210086 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b4ad45192769bbb99e22e58110c9dd8f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18069290 15163598 |
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