Synthetic extracellular matrices with tailored adhesiveness and degradability support lumen formation during angiogenic sprouting
| العنوان: | Synthetic extracellular matrices with tailored adhesiveness and degradability support lumen formation during angiogenic sprouting |
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| المؤلفون: | Britta Trappmann, Jifeng Liu, Dagmar Zeuschner, William J. Polacheck, Andreas F. B. Räder, Hongyan Long, Horst Kessler, Lydia Sorokin |
| المصدر: | Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021) Nature Communications |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Matrix remodeling, Angiogenesis, Science, Neovascularization, Physiologic, General Physics and Astronomy, 02 engineering and technology, Article, General Biochemistry, Genetics and Molecular Biology, In vitro model, 03 medical and health sciences, Tissue engineering, Lab-On-A-Chip Devices, Human Umbilical Vein Endothelial Cells, Extracellular, Humans, Sulfones, Cardiovascular models, Multidisciplinary, Tissue Engineering, Biomaterials - cells, Biomedical engineering, Chemistry, Adhesiveness, Dextrans, Hydrogels, General Chemistry, 021001 nanoscience & nanotechnology, Extracellular Matrix, ddc, Cell biology, Endothelial stem cell, 030104 developmental biology, Methacrylates, 0210 nano-technology, Lumen (unit), Sprouting |
| الوصف: | A major deficit in tissue engineering strategies is the lack of materials that promote angiogenesis, wherein endothelial cells from the host vasculature invade the implanted matrix to form new blood vessels. To determine the material properties that regulate angiogenesis, we have developed a microfluidic in vitro model in which chemokine-guided endothelial cell sprouting into a tunable hydrogel is followed by the formation of perfusable lumens. We show that long, perfusable tubes only develop if hydrogel adhesiveness and degradability are fine-tuned to support the initial collective invasion of endothelial cells and, at the same time, allow for matrix remodeling to permit the opening of lumens. These studies provide a better understanding of how cell-matrix interactions regulate angiogenesis and, therefore, constitute an important step towards optimal design criteria for tissue-engineered materials that require vascularization. Current tissue engineering strategies lack materials that promote angiogenesis. Here the authors develop a microfluidic in vitro model in which chemokine-guided endothelial cell sprouting into a tunable hydrogel is followed by the formation of perfusable lumens to determine the material properties that regulate angiogenesis. |
| وصف الملف: | application/pdf |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4b60cd7ce9cd41ca71d5e267d2654b4 https://doi.org/10.1038/s41467-021-23644-5 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b4b60cd7ce9cd41ca71d5e267d2654b4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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