Bidirectional astrocytic GLUT1 activation by elevated extracellular K +
| العنوان: | Bidirectional astrocytic |
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| المؤلفون: | L. F. Barros, Ignacio Fernández-Moncada, Robert Epp, Daniel Robles-Maldonado, Pablo Castro, Karin Alegría, Iván Ruminot |
| المصدر: | Glia. 69:1012-1021 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Glucose Transporter Type 1, Glucose transporter, 3-O-Methylglucose, Stimulation, Metabolism, Biology, Cell biology, Mice, 03 medical and health sciences, Cellular and Molecular Neuroscience, Glucose, 030104 developmental biology, 0302 clinical medicine, Neurology, Anaerobic glycolysis, Astrocytes, Extracellular, biology.protein, Animals, GLUT1, Glycolysis, Lactic Acid, 030217 neurology & neurosurgery |
| الوصف: | The acute rise in interstitial K+ that accompanies neural activity couples the energy demand of neurons to the metabolism of astrocytes. The effects of elevated K+ on astrocytes include activation of aerobic glycolysis, inhibition of mitochondrial respiration and the release of lactate. Using a genetically encoded FRET glucose sensor and a novel protocol based on 3-O-methylglucose trans-acceleration and numerical simulation of glucose dynamics, we report that extracellular K+ is also a potent and reversible modulator of the astrocytic glucose transporter GLUT1. In cultured mouse astrocytes, the stimulatory effect developed within seconds, engaged both the influx and efflux modes of the transporter, and was detected even at 1 mM incremental K+ . The modulation of GLUT1 explains how astrocytes are able to maintain their glucose pool in the face of strong glycolysis stimulation. We propose that the stimulation of GLUT1 by K+ supports the production of lactate by astrocytes and the timely delivery of glucose to active neurons. |
| تدمد: | 1098-1136 0894-1491 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4de36ff6601d4bcd7e7fcf84e737c69 https://doi.org/10.1002/glia.23944 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....b4de36ff6601d4bcd7e7fcf84e737c69 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10981136 08941491 |
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