N-cadherin expression level modulates integrin-mediated polarity and strongly impacts on the speed and directionality of glial cell migration
| العنوان: | N-cadherin expression level modulates integrin-mediated polarity and strongly impacts on the speed and directionality of glial cell migration |
|---|---|
| المؤلفون: | Florent Peglion, Marc Sanson, Emeline Camand, Naël Osmani, Sandrine Etienne-Manneville |
| المساهمون: | Equipe de recherche sur les relations matrice extracellulaire-cellules (ERRMECe), Fédération INSTITUT DES MATÉRIAUX DE CERGY-PONTOISE (I-MAT), Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine, Polarité cellulaire, Migration et cancer, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Immuno-Rhumatologie Moléculaire, Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM UMR_S975, Service de Génétique Cytogénétique et Embryologie [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), PEGLION, FLORENT, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) |
| المصدر: | Journal of Cell Science Journal of Cell Science, 2012, 125 (4), pp.844-857. ⟨10.1242/jcs.087668⟩ |
| بيانات النشر: | HAL CCSD, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Integrins, Time Factors, Cell, CDC42, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], MESH: Cadherins, MESH: Down-Regulation, MESH: Glioma, Cell Movement, Cell polarity, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Guanine Nucleotide Exchange Factors, MESH: Guanine Nucleotide Exchange Factors, Phosphorylation, cdc42 GTP-Binding Protein, MESH: Cell Movement, Confluency, Cell Polarity, MESH: Integrins, Glioma, MESH: Gene Expression Regulation, Neoplastic, Cadherins, Cell biology, Gene Expression Regulation, Neoplastic, medicine.anatomical_structure, MESH: Cell Polarity, MESH: Phosphotyrosine, MESH: Cell Line, Tumor, Proto-Oncogene Proteins pp60(c-src), Down-Regulation, Biology, Focal adhesion, Glial cell migration, Cell Line, Tumor, MESH: Rho Guanine Nucleotide Exchange Factors, medicine, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Humans, Phosphotyrosine, Focal Adhesions, Wound Healing, MESH: Humans, MESH: cdc42 GTP-Binding Protein, MESH: Phosphorylation, Cadherin, MESH: Time Factors, MESH: Focal Adhesions, Cell Biology, MESH: Proto-Oncogene Proteins pp60(c-src), MESH: Astrocytes, MESH: Wound Healing, MESH: Wounds and Injuries, Astrocytes, Cancer cell, Wounds and Injuries, Rho Guanine Nucleotide Exchange Factors |
| الوصف: | International audience; Perturbation of cell polarity is a hallmark of cancer cells. In carcinomas, loss of epithelial E-cadherin contributes to the loss of cell polarity and promotes epithelial–mesenchymal transition and carcinoma infiltration. However, the contribution of classical cadherins to the development of non-epithelial tumours is less well documented. We investigated the impact of the level of N-cadherin expression on the polarity and migration of normal and tumour glial cells. Low levels of N-cadherin were frequently observed in human glioma samples and purified glioma cells. Using a wound-healing assay, we show that a decreased level of N-cadherin promotes a faster and less-directed migration both in normal and tumour cells. N-cadherin-mediated contacts control cell velocity and polarity through the regulation of focal adhesions. In cells expressing low levels of N-cadherin, small focal adhesions are present at the entire cell periphery of confluent cells and are not affected by wounding of the cell monolayer. Under these conditions, wound-induced integrin-mediated recruitment of the small GTPase Cdc42, activation of the Cdc42-mediated polarity pathway and centrosome reorientation do not occur. Re-expression of N-cadherin in gliomas restores cell polarity and strongly reduces cell velocity, suggesting that loss of N-cadherin could contribute to the invasive capacity of tumour astrocytes. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0021-9533 1477-9137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b511c920ea35d8e43c20720a00b43e21 https://hal-pasteur.archives-ouvertes.fr/pasteur-03521577/file/844.pdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b511c920ea35d8e43c20720a00b43e21 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219533 14779137 |
|---|