A subtelomeric region affects telomerase-negative replicative senescence in Saccharomyces cerevisiae
| العنوان: | A subtelomeric region affects telomerase-negative replicative senescence in Saccharomyces cerevisiae |
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| المؤلفون: | Stephan Eberhard, Pascale Jolivet, Maria Teresa Teixeira, Kamar Serhal, Zhou Xu, Brian Luke, Marco Graf |
| المساهمون: | Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes (LBMCE), Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institute of Molecular Biology (IMB), Johannes Gutenberg - Universität Mainz (JGU), Physiologie membranaire et moléculaire du chloroplaste (PMMC), Sorbonne Université (SU)-Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Xu, Zhou, Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU) |
| المصدر: | Scientific Reports Scientific Reports, Nature Publishing Group, 2019, 9 (1), ⟨10.1038/s41598-018-38000-9⟩ Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) Scientific Reports, 2019, 9 (1), ⟨10.1038/s41598-018-38000-9⟩ |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Senescence, Telomerase, Saccharomyces cerevisiae Proteins, DNA repair, [SDV]Life Sciences [q-bio], Saccharomyces cerevisiae, lcsh:Medicine, Context (language use), Biology, Article, 03 medical and health sciences, 0302 clinical medicine, lcsh:Science, Cellular Senescence, Telomere Shortening, Multidisciplinary, lcsh:R, Cell Cycle, Recombinational DNA Repair, Telomere, biology.organism_classification, Subtelomere, Cell biology, [SDV] Life Sciences [q-bio], 030104 developmental biology, Chromosome Structures, Essential gene, lcsh:Q, 030217 neurology & neurosurgery, Cell Division |
| الوصف: | In eukaryotes, telomeres determine cell proliferation potential by triggering replicative senescence in the absence of telomerase. In Saccharomyces cerevisiae, senescence is mainly dictated by the first telomere that reaches a critically short length, activating a DNA-damage-like response. How the corresponding signaling is modulated by the telomeric structure and context is largely unknown. Here we investigated how subtelomeric elements of the shortest telomere in a telomerase-negative cell influence the onset of senescence. We found that a 15 kb truncation of the 7L subtelomere widely used in studies of telomere biology affects cell growth when combined with telomerase inactivation. This effect is likely not explained by (i) elimination of sequence homology at chromosome ends that would compromise homology-directed DNA repair mechanisms; (ii) elimination of the conserved subtelomeric X-element; (iii) elimination of a gene that would become essential in the absence of telomerase; and (iv) heterochromatinization of inner genes, causing the silencing of an essential gene in replicative senescent cells. This works contributes to better delineate subtelomere functions and their impact on telomere biology. |
| وصف الملف: | application/pdf |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b513f488d7e835043d19c6e4386efef7 https://pubmed.ncbi.nlm.nih.gov/30755624 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b513f488d7e835043d19c6e4386efef7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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