Yaws in West Sumatra, Indonesia: Clinical manifestations, serological findings and characterisation of new Treponema isolates by DNA probes
| العنوان: | Yaws in West Sumatra, Indonesia: Clinical manifestations, serological findings and characterisation of new Treponema isolates by DNA probes |
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| المؤلفون: | J. J. Van Der Sluis, J. Judanarso, J. Van Der Stek, J. D. A. Van Embden, G. T. Noordhoek, Ernst Stolz, G.M.N. Aelbers, H. J. H. Engelkens |
| المصدر: | European Journal of Clinical Microbiology & Infectious Diseases. 10:12-19 |
| بيانات النشر: | Springer Science and Business Media LLC, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | Adult, Male, Microbiology (medical), medicine.medical_specialty, Adolescent, Blotting, Western, Fluorescent Antibody Technique, Fluorescent treponemal antibody absorption test, Biology, Polymerase Chain Reaction, Serology, Immunoenzyme Techniques, Medical microbiology, Antigen, Prevalence, medicine, Humans, Treponema pallidum, Child, Skin, Hemagglutination assay, Treponema, medicine.diagnostic_test, Hemagglutination Tests, General Medicine, Middle Aged, biology.organism_classification, Virology, Infectious Diseases, Indonesia, Child, Preschool, Immunoassay, Yaws, biology.protein, Female, Antibody, DNA Probes |
| الوصف: | The results of a yaws survey on the island of Sumatra in Indonesia are presented. The prevalence of yaws in the investigated region was found to be very high, a minimum of 300 cases per 100,000 individuals, which indicates that yaws is far from being eradicated and that campaigns for treatment are necessary. Patients suffering from early infectious yaws showed florid skin lesions. Of 101 serum samples from such patients, 100 had a positive reaction in one or more treponemal tests. The Treponema pallidum haemagglutination assay was found to be the most sensitive test (97% positive) in detecting antibodies against Treponema pallidum subsp. pertenue, followed by the fluorescent treponemal antibody absorption test (94%), the Venereal Disease Research Laboratory test and the TmpA enzyme immunoassay (91%), and analysis by Western blot using Treponema pallidum antigens (88%). Of 42 asymptomatic contacts of yaws patients 32 showed positive reactions in one or more tests, indicating that many people in the investigated region have been infected with treponemes. Eight new Treponema pallidum subsp. pertenue strains were isolated from yaws skin lesions. In vitro amplification of treponemal DNA and hybridisation with specific DNA probes showed that all eight strains were identical with Treponema pallidum subsp. pertenue CDC 2575, with regard to the subsp. pertenue specific tyfl gene. |
| تدمد: | 1435-4373 0934-9723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b61d49c419e1cf3879ec285dc6b4f555 https://doi.org/10.1007/bf01967091 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....b61d49c419e1cf3879ec285dc6b4f555 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14354373 09349723 |
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