Cellular senescence in human myoblasts is overcome by human telomerase reverse transcriptase and cyclin-dependent kinase 4: consequences in aging muscle and therapeutic strategies for muscular dystrophies
| العنوان: | Cellular senescence in human myoblasts is overcome by human telomerase reverse transcriptase and cyclin-dependent kinase 4: consequences in aging muscle and therapeutic strategies for muscular dystrophies |
|---|---|
| المؤلفون: | Anne Bigot, James P. Di Santo, Vincent Mouly, Racquel N. Cooper, Woodring E. Wright, Jerry W. Shay, Chun Hong Zhu, Kamel Mamchaoui, Gillian Butler-Browne |
| المساهمون: | University of Texas Southwestern Medical Center [Dallas], Groupe Myologie, Institut de Myologie, Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cytokines et Développement Lymphoïde, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), The authors wish to acknowledge their financial support: AFM (Association Française contre les Myopathies), INSERM, Université Pierre et Marie Curie, CNRS, the MYORES Network of Excellence – contract 511978 and the MYOAMP STREP, both from the European Commission 6th Framework Programme, and AG07992 from the National Institute on Aging. Woodring E. Wright is an Ellison Medical Foundation Senior Scholar., European Project: 38902,MYOAMP, Bigot, Anne, Amplification of human myogenic stem cells in clinical conditions - MYOAMP - 38902 - OLD |
| المصدر: | Aging Cell Aging Cell, 2007, 6 (4), pp.515-523. ⟨10.1111/j.1474-9726.2007.00306.x⟩ |
| بيانات النشر: | HAL CCSD, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | muscular dystrophy, Telomerase, Aging, satellite stem cell, Satellite Cells, Skeletal Muscle, [SDV]Life Sciences [q-bio], Transplantation, Heterologous, Clone (cell biology), p16, Biology, Muscle Development, telomerase, Muscular Dystrophies, Cell Line, Myoblasts, Mice, replicative senescence, Myocyte, Animals, Humans, Regeneration, Telomerase reverse transcriptase, replicative lifespan, Cellular Senescence, Cyclin-dependent kinase 4, Regeneration (biology), Muscles, Cyclin-Dependent Kinase 4, Cell Biology, Telomere, Molecular biology, Cell biology, [SDV] Life Sciences [q-bio], Transplantation, Genes, cdc, biology.protein, myopathies, hTERT, Cell Division |
| الوصف: | International audience; Cultured human myoblasts fail to immortalize following the introduction of telomerase. The availability of an immortalization protocol for normal human myoblasts would allow one to isolate cellular models from various neuromuscular diseases, thus opening the possibility to develop and test novel therapeutic strategies. The parameters limiting the efficacy of myoblast transfer therapy (MTT) could be assessed in such models. Finally, the presence of an unlimited number of cell divisions, and thus the ability to clone cells after experimental manipulations, reduces the risks of insertional mutagenesis by many orders of magnitude. This opportunity for genetic modification provides an approach for creating a universal donor that has been altered to be more therapeutically useful than its normal counterpart. It can be engineered to function under conditions of chronic damage (which are very different than the massive regeneration conditions that recapitulate normal development), and to overcome the biological problems such as cell death and failure to proliferate and migrate that limit current MTT strategies. We describe here the production and characterization of a human myogenic cell line, LHCN-M2, that has overcome replicative aging due to the expression of telomerase and cyclin-dependent kinase 4. We demonstrate that it functions as well as young myoblasts in xenotransplant experiments in immunocompromized mice under conditions of regeneration following muscle damage. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1474-9718 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b67e2d0bfb61f4e54a0ec11c5648358b https://hal.science/hal-03833731/document |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b67e2d0bfb61f4e54a0ec11c5648358b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14749718 |
|---|