Two types of O‐methyltransferase are involved in biosynthesis of anticancer methoxylated 4′‐deoxyflavones in Scutellaria baicalensis Georgi
| العنوان: | Two types of O‐methyltransferase are involved in biosynthesis of anticancer methoxylated 4′‐deoxyflavones in Scutellaria baicalensis Georgi |
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| المؤلفون: | Yumin Fang, Jun Yang, Yu-Kun Wei, Jianxu Li, Xin Zhong, Qing Zhao, Jie Liu, Tianlin Pei, Xiao-Ya Chen, Yonghong Hu, Meng-Ying Cui, An-Rui Lu, Yu Kong, Cathie Martin, Wen-Qing Qiu |
| المصدر: | Plant Biotechnology Journal |
| بيانات النشر: | John Wiley and Sons Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Plant Science, Chrysoeriol, Flavones, Plant Roots, chemistry.chemical_compound, flavones, Biosynthesis, Research Articles, O‐methyltransferase, chemistry.chemical_classification, Flavonoids, Plants, Medicinal, biology, Methyltransferases, biology.organism_classification, O-methyltransferase, Baicalein, chemistry, Biochemistry, biology.protein, Scutellaria, Scutellaria baicalensis, Oroxylin A, biosynthesis, Agronomy and Crop Science, Biotechnology, Research Article |
| الوصف: | Summary The medicinal plant Scutellaria baicalensis Georgi is rich in specialized 4′‐deoxyflavones, which are reported to have many health‐promoting properties. We assayed Scutellaria flavones with different methoxyl groups on human cancer cell lines and found that polymethoxylated 4′‐deoxyflavones, like skullcapflavone I and tenaxin I have stronger ability to induce apoptosis compared to unmethylated baicalein, showing that methoxylation enhances bioactivity as well as the physical properties of specialized flavones, while having no side‐effects on healthy cells. We investigated the formation of methoxylated flavones and found that two O‐methyltransferase (OMT) families are active in the roots of S. baicalensis. The Type II OMTs, SbPFOMT2 and SbPFOMT5, decorate one of two adjacent hydroxyl groups on flavones and are responsible for methylation on the C6, 8 and 3′‐hydroxyl positions, to form oroxylin A, tenaxin II and chrysoeriol respectively. The Type I OMTs, SbFOMT3, SbFOMT5 and SbFOMT6 account mainly for C7‐methoxylation of flavones, but SbFOMT5 can also methylate baicalein on its C5 and C6‐hydroxyl positions. The dimethoxylated flavone, skullcapflavone I (found naturally in roots of S. baicalensis) can be produced in yeast by co‐expressing SbPFOMT5 plus SbFOMT6 when the appropriately hydroxylated 4′‐deoxyflavone substrates are supplied in the medium. Co‐expression of SbPFOMT5 plus SbFOMT5 in yeast produced tenaxin I, also found in Scutellaria roots. This work showed that both type I and type II OMT enzymes are involved in biosynthesis of methoxylated flavones in S. baicalensis. |
| اللغة: | English |
| تدمد: | 1467-7652 1467-7644 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6b1eee4011c1493abaf7977a0d21ec2 http://europepmc.org/articles/PMC8710825 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b6b1eee4011c1493abaf7977a0d21ec2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14677652 14677644 |
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