Reduced brain edema and functional deficits after treatment of diffuse traumatic brain injury by carbamylated erythropoietin derivative
| العنوان: | Reduced brain edema and functional deficits after treatment of diffuse traumatic brain injury by carbamylated erythropoietin derivative |
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| المؤلفون: | Samuel Valable, Gilles Francony, Jean François Payen, Pierre Bouzat, Hana Lahrech, Marie Cécile Fevre, Franck Mauconduit, Myriam Bernaudin, Sébastien Thomas, Emmanuel L. Barbier |
| المساهمون: | INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale, Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Service d'anesthésie-réanimation, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-Hôpital Michallon-CHU Grenoble-Hôpital Michallon, Centre-Imagerie, Neurosciences, et Application aux Pathologies (CI-NAPS - UMR 6232), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Inserm U836, équipe 7, Nanomédecine et cerveau, Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Grenoble Institut des Neurosciences ( GIN ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Grenoble-Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Grenoble-Université Joseph Fourier - Grenoble 1 ( UJF ) -Service d'anesthésie-réanimation, Université Joseph Fourier - Grenoble 1 ( UJF ) -CHU Grenoble-Hôpital Michallon-Hôpital Michallon, Centre-Imagerie, Neurosciences, et Application aux Pathologies ( CI-NAPS - UMR 6232 ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Grenoble-Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -CHU Grenoble-Université Joseph Fourier - Grenoble 1 ( UJF ), Neuro-imagerie fonctionnelle et métabolique ( ANTE-INSERM U836, équipe 5 ), Dojat, Michel |
| المصدر: | Critical Care Medicine Critical Care Medicine, Lippincott, Williams & Wilkins, 2011, 39 (9), pp.2099-105. ⟨10.1097/CCM.0b013e31821cb7b2⟩ Critical Care Medicine, 2011, 39 (9), pp.2099-105. ⟨10.1097/CCM.0b013e31821cb7b2⟩ Critical Care Medicine, Lippincott, Williams & Wilkins, 2011, 39 (9), pp.2099-105. 〈10.1097/CCM.0b013e31821cb7b2〉 |
| بيانات النشر: | HAL CCSD, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Male, MESH : Reflex, Time Factors, MESH: Brain Edema, medicine.medical_treatment, MESH : Psychomotor Disorders, Brain Edema, Critical Care and Intensive Care Medicine, MESH: Magnetic Resonance Imaging, 0302 clinical medicine, Edema, MESH : Erythropoietin, MESH: Animals, Saline, 0303 health sciences, MESH : Brain Injuries, medicine.diagnostic_test, MESH : Rats, Brain, Magnetic Resonance Imaging, Anesthesia, MESH: Psychomotor Disorders, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Brain Edema, medicine.symptom, MESH : Time Factors, medicine.drug, MESH: Rats, Traumatic brain injury, MESH: Reflex, MESH : Male, MESH : Rats, Wistar, MESH : Cognition Disorders, 03 medical and health sciences, MESH: Brain, MESH : Magnetic Resonance Imaging, Reflex, medicine, Animals, Effective diffusion coefficient, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Erythropoietin, Rats, Wistar, Erythropoietin, 030304 developmental biology, business.industry, MESH: Time Factors, Magnetic resonance imaging, MESH: Rats, Wistar, medicine.disease, MESH: Male, Rats, Carbamylated erythropoietin, MESH: Cognition Disorders, MESH : Brain, Brain Injuries, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH: Brain Injuries, MESH : Animals, Psychomotor Disorders, Cognition Disorders, business, 030217 neurology & neurosurgery |
| الوصف: | International audience; OBJECTIVE: To investigate the effects of carbamylated erythropoietin, a modified erythropoietin lacking erythropoietic activity, on brain edema and functional recovery in a model of diffuse traumatic brain injury. DESIGN: Adult male Wistar rats. SETTING: Neurosciences and physiology laboratories. INTERVENTIONS: Thirty minutes after diffuse traumatic brain injury (impact-acceleration model), rats were intravenously administered with either a saline solution (traumatic brain injury-saline) or carbamylated erythropoietin (50 μg/kg; traumatic brain injury-carbamylated erythropoietin). A third group received no traumatic brain injury insult (sham-operated). MEASUREMENTS AND MAIN RESULTS: Three series of experiments were conducted to investigate: 1) the effect of carbamylated erythropoietin on brain edema before and 1 hr after traumatic brain injury using diffusion-weighted magnetic resonance imaging and measurements of apparent diffusion coefficient (n = 10 rats per group), and the phosphorylation level of brain extracellular-regulated kinase-1/-2 was also determined to indicate the presence of an activated cell signaling pathway; 2) the time course of brain edema using magnetic resonance imaging between 4 and 6 hrs postinjury and the gravimetric technique at 6 hrs (n = 10 rats per group); and 3) motor and cognitive function over 10 days post traumatic brain injury, testing acute somatomotor reflexes, adhesive paper removal, and two-way active avoidance (n = 8 rats per group). Compared to traumatic brain injury-saline rats, rats receiving traumatic brain injury-carbamylated erythropoietin showed a significant reduction in brain edema formation at 1 hr that was sustained until 6 hrs when results were comparable with sham-operated rats. This antiedematous effect of carbamylated erythropoietin was possibly mediated through an early inhibition of extracellular-regulated kinase-1/-2 phosphorylation. Compared to traumatic brain injury-saline rats, traumatic brain injury-carbamylated erythropoietin rats showed improved functional recovery of the acute somatomotor reflexes post traumatic brain injury, took less time to remove adhesive from the forelimbs, and showed higher percentages of correct avoidance responses. CONCLUSION: Our findings indicate that early posttraumatic administration of carbamylated erythropoietin reduces brain edema development until at least 6 hrs postinjury and improves neurologic recovery. Carbamylated erythropoietin can thus be considered as a potential agent in the treatment of traumatic brain injury-induced diffuse edema. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0090-3493 1530-0293 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6bff5bc4e8fe52decfcc85b22a977a1 https://www.hal.inserm.fr/inserm-00658345 |
| حقوق: | EMBARGO |
| رقم الأكسشن: | edsair.doi.dedup.....b6bff5bc4e8fe52decfcc85b22a977a1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00903493 15300293 |
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