Stage specific gene expression of serpins and their cognate proteases during myeloid differentiation
| العنوان: | Stage specific gene expression of serpins and their cognate proteases during myeloid differentiation |
|---|---|
| المؤلفون: | Melinda Anne Missen, Robert L. Medcalf, Paul Bernard Coughlin, Genevieve Whitty, David N. Haylock |
| المصدر: | British Journal of Haematology. 135:715-724 |
| بيانات النشر: | Wiley, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Proteases, Time Factors, medicine.medical_treatment, Cellular differentiation, Gene Expression, Bone Marrow Cells, Biology, Serpin, Cell Line, Plasminogen Activator Inhibitor 2, medicine, Humans, Cell Lineage, Cells, Cultured, Serpins, Leukemia, Protease, Reverse Transcriptase Polymerase Chain Reaction, Elastase, Cell Differentiation, Hematology, Hematopoietic Stem Cells, Cell biology, Haematopoiesis, alpha 1-Antitrypsin, Neutrophil elastase, Immunology, biology.protein, Myelopoiesis, Peptide Hydrolases |
| الوصف: | Proteases and their serpin inhibitors are abundantly expressed in haemopoietic and peripheral blood cells. There is, however, relatively little information about the role played by serpins in the control of protease activity within these cells and in the pericellular region. The observation that mutations in the neutrophil elastase gene, which cause cyclic and severe congenital neutropenia, are associated with protease maldistribution gives some clue as to the potential importance of inhibitor proteins. To begin to address the role of protease/inhibitor balance in blood cells we used reverse transcription polymerase chain reaction to examine protease and serpin gene expression in mature peripheral blood cells, differentiating haemopoietic progenitors, leukaemic blasts and haemopoietic cell lines. The results demonstrate stage-specific expression of proteases together with widespread expression of intra- and extra-cellular serpins. The elastase inhibitors monocyte neutrophil elastase inhibitor (MNEI) and antitrypsin (AT) showed overlapping expression. MNEI is predominantly expressed in early haemopoietic progenitors while antitrypsin is mainly expressed in more mature myeloid precursors, peripheral blood granulocytes and mononuclear cells. Our results give an overall picture of serpin and protease gene expression and draws attention to the potential importance of elastase regulators at all stages of myelopoiesis. |
| تدمد: | 1365-2141 0007-1048 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b707869e66981dcfec3d2d8c827daa3f https://doi.org/10.1111/j.1365-2141.2006.06360.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....b707869e66981dcfec3d2d8c827daa3f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652141 00071048 |
|---|