Histopathological study of the maternal exposure to the biologically produced silver nanoparticles on different organs of the offspring
| العنوان: | Histopathological study of the maternal exposure to the biologically produced silver nanoparticles on different organs of the offspring |
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| المؤلفون: | Parastoo Pourali, Faezeh Ameri, Tana Heidari, Behrooz Yahyaei, Sepideh Arabzadeh, Niloufar Kheirkhahan, Mahnaz Nouri |
| المصدر: | Naunyn-Schmiedeberg's Archives of Pharmacology. 393:867-878 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Offspring, Placenta, medicine.medical_treatment, Metal Nanoparticles, Ovary, 02 engineering and technology, Kidney, Andrology, Inhibitory Concentration 50, Mice, 03 medical and health sciences, chemistry.chemical_compound, Pregnancy, In vivo, Testis, medicine, Animals, Placental Circulation, Tissue Distribution, MTT assay, Rats, Wistar, Saline, IC50, 030304 developmental biology, Pharmacology, 0303 health sciences, Dose-Response Relationship, Drug, Silver Compounds, General Medicine, 021001 nanoscience & nanotechnology, Silver nitrate, medicine.anatomical_structure, Animals, Newborn, Liver, chemistry, Maternal Exposure, NIH 3T3 Cells, Female, 0210 nano-technology |
| الوصف: | This research for the first time presents the possibility of crossing the biologically produced SNPs through the placenta to different organs of rat offspring. SNPs were produced using Fusarium oxysporum. After adding 1 mmol final concentration of silver nitrate solution to the culture supernatant and 5 min heating, SNPs were produced, and their production was proved using visible spectrum, transmission electron microscope (TEM), and X-ray diffraction (XRD) analyses. SNPs were washed, and their concentration determined using inductively coupled plasma (ICP) instrument. SNPs were used for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and after determination of their half maximal inhibitory concentration (IC50) dose, their toxic and nontoxic doses were determined and used for in vivo studies. A total of 24 female rats, after detection of their vaginal plugs, were divided into 3 groups each having 8 members. A control group was treated with normal saline. The other two groups were treated by toxic and nontoxic doses of SNPs, respectively. After delivery and breastfeeding, the pups were scarified, and their organs were collected and analyzed using histological examinations. Results showed that SNPs had a maximum absorbance peak around 450 nm, with polygonal and round shapes. XRD results confirmed the presence of SNPs. The concentration of the SNPs after washing was 19 ppm/mL based on the ICP results. MTT assay results showed that SNPs had a dose-dependent toxic effect. Histopathological examination results showed that SNPs could pass through the placenta; both their nontoxic and toxic doses induced somehow mild alternations in the liver, kidney, testis, and ovary and had no effects on the brains of the rat offspring. In conclusions, the use of the biologically produced SNPs should be limited during pregnancy and breastfeeding. |
| تدمد: | 1432-1912 0028-1298 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b71835bc8554a9ef8b8604f7a6375ef4 https://doi.org/10.1007/s00210-019-01796-y |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....b71835bc8554a9ef8b8604f7a6375ef4 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14321912 00281298 |
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