LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis
| العنوان: | LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis |
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| المؤلفون: | Ling Zhang, Min Zhang, Congyu Li, Yunjian Wang, Luyang Zhang, Guohua You, Minghe Zhou, Bo Meng |
| المصدر: | Cancer Cell International Cancer Cell International, Vol 19, Iss 1, Pp 1-12 (2019) |
| بيانات النشر: | BioMed Central, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Cancer Research, Cell, LINC01006, miR-2682-5p, lcsh:RC254-282, Metastasis, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Western blot, Pancreatic cancer, Genetics, medicine, lcsh:QH573-671, Transcription factor, 030304 developmental biology, 0303 health sciences, Gene knockdown, medicine.diagnostic_test, Cell growth, Chemistry, lcsh:Cytology, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.anatomical_structure, Oncology, HOXB8, 030220 oncology & carcinogenesis, Cancer research, PC, Primary Research |
| الوصف: | Background Pancreatic cancer (PC) is one of the deadliest cancers about the digestive system. Recent researches have validated that long non-coding RNAs (lncRNAs) play vital roles in various cancers, while the function of LINC01006 in PC is rarely clarified. Aim of the study Investigation of the specific role of LINC01006 in PC. Methods LINC01006 expression was examined by RT-qPCR. CCK-8, EdU, transwell, wound healing, and western blot assays were carried out to explore the function of LINC01006 in PC. The interaction among LINC01006, miR-2682-5p and HOXB8 was verified by luciferase reporter, RIP and ChIP assays. Results The expression of LINC01006 was markedly upregulated in PC tissues and cells. Furthermore, LINC01006 knockdown inhibited PC cell proliferation, invasion and migration, and upregulation of LINC01006 led to the opposite results. Besides, miR-2682-5p expression was downregulated and negatively regulated by LINC01006 in PC. Meanwhile, LINC01006 could bind with miR-2682-5p in PC. Moreover, miR-2682-5p negatively regulated HOXB8 expression and there was a binding site between miR-2682-5p and HOXB8 in PC. Additionally, miR-2682-5p overexpression or HOXB8 knockdown rescued the promotive effects of LINC01006 upregulation on PC cell progression. Similarly, miR-2682-5p inhibition or HOXB8 overexpression countervailed the repressive role of LINC01006 downregulation in PC cell progression. In addition, the transcription factor HOXB8 could activate LINC01006 transcription in PC. Conclusions LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis, which may facilitate the treatment for PC. |
| اللغة: | English |
| تدمد: | 1475-2867 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b734f9ac814ac36cd5eb2a96d7ecdb45 http://europepmc.org/articles/PMC6889337 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b734f9ac814ac36cd5eb2a96d7ecdb45 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14752867 |
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