Bacterial immunogenic α-galactosylceramide identified in the murine large intestine: dependency on diet and inflammation
| العنوان: | Bacterial immunogenic α-galactosylceramide identified in the murine large intestine: dependency on diet and inflammation |
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| المؤلفون: | Roger Sandhoff, Hermann-Josef Gröne, Robert Pilz, Bruno Galy, Carsten Hopf, Valentin Sencio, Dominic Lamprecht, Daphnée Soulard, Johanna von Gerichten, Silke Herzer, Lukáš Opálka, Christian Marsching, Viola Nordström, François Trottein |
| المساهمون: | German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg] (DKFZ), University of Heidelberg, Medical Faculty, Faculty of Medicine in Hradec Kralove [Republique Tchèque], Charles University [Prague] (CU), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Center for Mass Spectrometry and Optical Spectroscopy [Mannheim, Germany] (CeMOS), Hochschule Augsburg - University of Applied Sciences, Philipps University of Marburg, This work was supported in part by the Institut National de la Santé et de la Recherche Médicale, the Centre National de la Recherche Scientifique, the University of Lille, the Pasteur Institute of Lille, and l’Agence Nationale de la Recherche (AAP générique 2017, ANR-17-CE15-0020-01, ACROBAT) (F.T.). S.H. received funding from Deutsche Forschungsgemeinschaft Grant HE 7978/1-1., ANR-17-CE15-0020,ACROBAT,Rôle de l'axe poumon/intestin/moelle osseuse et du microbiote au cours de la grippe(2017), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Philipps Universität Marburg = Philipps University of Marburg |
| المصدر: | Journal of Lipid Research, Vol 60, Iss 11, Pp 1892-1904 (2019) Journal of Lipid Research Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 2019, 60 (11), pp.1892-1904. ⟨10.1194/jlr.RA119000236⟩ Journal of Lipid Research, 2019, 60 (11), pp.1892-1904. ⟨10.1194/jlr.RA119000236⟩ J Lipid Res |
| بيانات النشر: | Elsevier, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, glycolipids, experimental colitis, Galactosylceramides, Mice, Inbred Strains, QD415-436, 030204 cardiovascular system & hematology, Gut flora, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Biochemistry, Microbiology, Bacteroides fragilis, immunology, Mice, 03 medical and health sciences, Cecum, 0302 clinical medicine, Endocrinology, Immune system, medicine, Animals, influenza A virus, invariant natural killer T cells, Intestine, Large, Microbiome, Western diet, bacteria, mass spectrometry, Inflammation, sphingolipids, biology, Chemistry, cluster of differentiation 1d, Cell Biology, biology.organism_classification, medicine.disease, Diet, 3. Good health, 030104 developmental biology, medicine.anatomical_structure, CD1D, Commentary, biology.protein, Cell activation, Dysbiosis |
| الوصف: | International audience; The glycosphingolipid, α-galactosylceramide (αGalCer), when presented by CD1d on antigen-presenting cells, efficiently activates invariant natural killer T (iNKT) cells. Thereby, it modulates immune responses against tumors, microbial and viral infections, and autoimmune diseases. Recently, the production of αGalCer by Bacteroidetes from the human gut microbiome was elucidated. Using hydrophilic interaction chromatography coupled to MS2, we screened murine intestinal tracts to identify and quantify αGalCers, and we investigated the αGalCer response to different dietary and physiologic conditions. In both the cecum and the colon of mice, we found 1-15 pmol of αGalCer per milligram of protein; in contrast, mice lacking microbiota (germ-free mice) and fed identical diet did not harbor αGalCer. The identified αGalCer contained a β(R)-hydroxylated hexadecanoyl chain N-linked to C18-sphinganine, which differed from what has been reported with Bacteroides fragilis Unlike β-anomeric structures, but similar to αGalCers from B. fragilis, the synthetic form of the murine αGalCer induced iNKT cell activation in vitro. Last, we observed a decrease in αGalCer production in mice exposed to conditions that alter the composition of the gut microbiota, including Western type diet, colitis, and influenza A virus infection. Collectively, this study suggests that αGalCer is produced by commensals in the mouse intestine and reveals that stressful conditions causing dysbiosis alter its synthesis. The consequences of this altered production on iNKT cell-mediated local and systemic immune responses are worthy of future studies. |
| اللغة: | English |
| تدمد: | 0022-2275 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b75689cc708adf4690d205d375f9fe9d http://www.sciencedirect.com/science/article/pii/S0022227520322872 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b75689cc708adf4690d205d375f9fe9d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00222275 |
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