Biological Characteristics and Carrier Functions of Pegylated Manganese Zinc Ferrite Nanoparticles
| العنوان: | Biological Characteristics and Carrier Functions of Pegylated Manganese Zinc Ferrite Nanoparticles |
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| المؤلفون: | Yujuan Shi, Fengxiao Dou, Xiaoqian Feng, Xingmao Jiang, Chenglin Zhou, Xuefeng Bian, Ting Guo, Jun Zhang, Mei Lin, Ning Xu, Yanhong Xiao, Junxing Huang, Hong Yu, Jun Ye |
| المصدر: | Journal of Nanomaterials, Vol 2019 (2019) |
| بيانات النشر: | Hindawi Limited, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Materials science, Article Subject, Biocompatibility, technology, industry, and agriculture, chemistry.chemical_element, Nanoparticle, 02 engineering and technology, Transfection, Manganese, 021001 nanoscience & nanotechnology, In vitro, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Plasmid, chemistry, 030220 oncology & carcinogenesis, lcsh:Technology (General), Biophysics, lcsh:T1-995, General Materials Science, MTT assay, 0210 nano-technology, DNA |
| الوصف: | This study was performed to investigate the biocompatibility (BC), magnetothermal effect, and DNA binding biological characteristics of manganese zinc ferrite nanoparticles (Mn0.6Zn0.4Fe2O4-NPs (MZF-NPs)) coated with pegylated manganese (PEG-MZF-NPs). Their functions as gene transfer carrier for gene therapy and magnetic medium for tumor hyperthermia were also explored. The manganese zinc ferrite nanoparticles were synthesized through high temperature cracking, and their characterizations were discovered. Hemolysis test and MTT assay were performed to evaluate biocompatibility, and their self-heating effects in the alternating magnetic field were investigated. PEG-MZF-NPs with different concentrations were measured by using 7.0 T Micro-MR scanner (MRI) to calculate the T2 value and r2 relaxation rate of each sample. The CD44-shRNA plasmids were constructed, and their ability to bind PEG-MZF-NPs were examined. The DNA release from PEG-MZF-NP/DNA complex and protection of DNA from nuclease digestion were also detected. After CD44-shRNA-EGFP were transfected into the ovarian cancer SK-OV-3 cells by using PEG-MZF-NPs as carriers, the transfection efficiency was detected by a flow cytometer and expression of CD44 mRNA and protein in cells was detected using RT-PCR and Western blot, respectively. We successfully prepared PEG-MZF-NPs with favorable dispersity, magnetic responsiveness, and BC. Typically, the excellent magnetothermal effect can be used for a tumor magnetothermal therapeutic study. In vitro MRI showed the application potential for being magnetic resonance T2 relaxation contrast agents and the possibility to achieve goal of integration of targeting diagnosis and treatment. The CD44-shRNA plasmids have been successfully constructed and concluded that PEG-MZF-NPs may serve as gene transfer carriers for gene therapy. |
| وصف الملف: | text/xhtml |
| تدمد: | 1687-4129 1687-4110 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b76ea0b1201caea5225f7e644240e43f https://doi.org/10.1155/2019/6854710 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b76ea0b1201caea5225f7e644240e43f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16874129 16874110 |
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