Morphological difference between pleural mesothelioma cells in effusion smears with either BAP1 loss or 9p21 homozygous deletion and reactive mesothelial cells without the gene alterations
| العنوان: | Morphological difference between pleural mesothelioma cells in effusion smears with either BAP1 loss or 9p21 homozygous deletion and reactive mesothelial cells without the gene alterations |
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| المؤلفون: | Kunimitsu Kawahara, Kazuki Nabeshima, Toshiaki Kamei, Yoshiaki Kinoshita, Makoto Hamasaki, Shinji Matsumoto |
| المصدر: | Pathology International. 69:637-645 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Mesothelioma, 0301 basic medicine, Pathology, medicine.medical_specialty, Pleural Neoplasms, Reactive mesothelial cells, Immunocytochemistry, Biology, Pathology and Forensic Medicine, 03 medical and health sciences, 0302 clinical medicine, Biomarkers, Tumor, medicine, Humans, Gene, Aged, Sequence Deletion, Aged, 80 and over, BAP1, medicine.diagnostic_test, Pleural mesothelioma, Tumor Suppressor Proteins, General Medicine, Middle Aged, Gene deletion, Immunohistochemistry, 030104 developmental biology, Effusion, 030220 oncology & carcinogenesis, Female, Chromosome Deletion, Chromosomes, Human, Pair 9, Ubiquitin Thiolesterase, Fluorescence in situ hybridization |
| الوصف: | We previously characterized the morphological characteristics of malignant pleural mesothelioma (MPM) cells with 9p21 homozygous deletion (HD) using a combination of the virtual microscopy and fluorescence in situ hybridization (FISH). In this study, we investigated whether MPM cells with BRCA1-associated protein 1 (BAP1) loss show the same morphological characteristics identified in MPM cells with 9p21 HD. MPM cells with either BAP1 loss detected by immunocytochemistry (ICC) or 9p21 HD detected by FISH were identified via virtual microscopy prior to ICC or FISH, followed by analysis and quantification of their morphological characteristics. MPM cells with BAP1 loss or 9p21 HD exhibited significantly more frequent cell-in-cell engulfment, multinucleation, and larger multicellular clusters composed of more than 10 cells than reactive mesothelial cells. In conclusion, MPM cells with BAP1 loss or 9p21 HD share similar cytological features, indicating that the same morphological criteria can be used to detect MPM cells harboring such genetic aberrations. |
| تدمد: | 1440-1827 1320-5463 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b77c2ac86c414e0e5970e6f5fec502e8 https://doi.org/10.1111/pin.12862 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....b77c2ac86c414e0e5970e6f5fec502e8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14401827 13205463 |
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