Diversification of Clonal Complex 5 Methicillin-Resistant Staphylococcus aureus Strains (Rhine-Hesse Clone) within Germany
| العنوان: | Diversification of Clonal Complex 5 Methicillin-Resistant Staphylococcus aureus Strains (Rhine-Hesse Clone) within Germany |
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| المؤلفون: | Christiane Wolz, Gabriele Bierbaum, Berit Schulte, Konstanze Pohl, Christiane Goerke |
| المصدر: | Journal of Clinical Microbiology. 51:212-216 |
| بيانات النشر: | American Society for Microbiology, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Methicillin-Resistant Staphylococcus aureus, Microbiology (medical), Genetics, Molecular Epidemiology, Genotype, Molecular epidemiology, Epidemiology, Genetic Variation, Staphylococcal Infections, Biology, Staphylococcal infections, medicine.disease, medicine.disease_cause, Methicillin-resistant Staphylococcus aureus, Microbiology, Staphylococcus aureus, Germany, medicine, Pulsed-field gel electrophoresis, Cluster Analysis, Humans, Multilocus sequence typing, Typing, Multilocus Sequence Typing, Phage typing |
| الوصف: | Since 1995, a methicillin-resistant Staphylococcus aureus (MRSA) clone has spread in southern Germany. The strain was assigned to the Rhine-Hesse pulsed-field gel electrophoresis (PFGE) type by the staphylococcal reference center and was highly similar to epidemic clones known to belong to clonal complex 5 (CC5; USA100) based on multilocus sequence typing (MLST). Here we analyzed a defined collection of strains assigned to the Rhine-Hesse/USA100 PFGE type. Using sequence-based typing methods (MLST, spa ), the isolates were divided into two distinct clusters, ST5 and its single-locus variant ST225. These two lineages are not distinguishable by PFGE or phage typing. Most of the ST5 isolates were derived from patients and volunteers from the Tübingen area in southwest Germany, whereas the ST225 isolates were mostly from other locations in Germany. The locally restricted ST5 isolates were shown to contain different SSC mec islands and exhibited different antibiotic resistance profiles. In contrast, the ST225 isolates form a highly homogenous group and are emerging all over Germany. The two lineages are clearly distinguishable by their phage content and spa type: ST5 strains from Tübingen are characterized by a Sa7int phage that carries the virulence gene sak , which codes for staphylokinase, and ST225 isolates are characterized by a Sa1int phage. In conclusion, based on sequence typing and phage content, CC5 strains can be subdivided into two distinct lineages with different epidemicities. |
| تدمد: | 1098-660X 0095-1137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b799b5f02e2ad6bdc7ef51a89aca7537 https://doi.org/10.1128/jcm.01967-12 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b799b5f02e2ad6bdc7ef51a89aca7537 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098660X 00951137 |
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