Chemical activation of the mechanotransduction channel Piezo1
| العنوان: | Chemical activation of the mechanotransduction channel Piezo1 |
|---|---|
| المؤلفون: | Jianmin Lao, Michael Bandell, H. Michael Petrassi, David C. Tully, Truc Huynh, Adrienne E. Dubin, Jie Xu, Jason T. Matzen, Jayanti Mathur, Mauricio Montal, Ruhma Syeda, Bertrand Coste, Andrew M. Schumacher, Ardem Patapoutian, Ingo H. Engels |
| المساهمون: | Laboratoire de Neurosciences Cognitives [Marseille] (LNC), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | eLife, Vol 4 (2015) eLife eLife, eLife Sciences Publication, 2015, 4, ⟨10.7554/eLife.07369⟩ eLife, 2015, 4, ⟨10.7554/eLife.07369⟩ |
| بيانات النشر: | eLife Sciences Publications Ltd, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | QH301-705.5, [SDV]Life Sciences [q-bio], Science, General Biochemistry, Genetics and Molecular Biology, Cell membrane, medicine, Mechanotransduction, Biology (General), agonist, Ion channel, mechanotransduction, General Immunology and Microbiology, Artificial cell, Chemistry, General Neuroscience, PIEZO1, Small Molecule Libraries, General Medicine, Anatomy, medicine.anatomical_structure, Membrane, Structural biology, ion channel, Biophysics, Medicine |
| الوصف: | Within our bodies, cells and tissues are constantly being pushed and pulled by their surrounding environment. These mechanical forces are then transformed into electrical or chemical signals by cells. This process is crucial for many biological structures, such as blood vessels, to develop correctly, and is also a key part of our senses of touch and hearing. In 2010, researchers discovered a group of ion channels—proteins embedded in the membrane that surrounds a cell—that open up when a force is applied and allow ions such as calcium, potassium, and sodium to flow. This movement of ions generates the electrical response of the cell to the applied force. However, not much is known about how these ‘Piezo’ ion channels work. To investigate this, it is important to be able to precisely control how and when the Piezo channels open. Many other ion channels are studied by using small chemical compounds to activate them, but there were none that were known to act on Piezo proteins. Syeda et al.—including some of the researchers involved in the 2010 work—screened over three million compounds for their ability to cause calcium ions to flow into human cells to try to identify chemicals that activate the Piezo channels. This revealed one promising candidate named Yoda1, which specifically activated Piezo1: a Piezo protein that had previously been linked to a role in blood vessel development in embryos. To investigate how Yoda1 activates Piezo1, Syeda et al. placed Piezo1 in an artificial cell membrane that did not contain any other cellular components. When Yoda1 was added to this set up, the Piezo1 channels opened up. This suggests that Piezo1 and Yoda1 interact in a manner that does not require additional cellular components other than a cell membrane. Separate work by Cahalan, Lukacs et al. uses Yoda1 to reveal that Piezo1 helps to control the volume of red blood cells, showing that in the future, Yoda1 could be valuable in research that investigates the roles of Piezo1. |
| اللغة: | English |
| تدمد: | 2050-084X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7ea2dc5b561868248bd11761649e432 https://elifesciences.org/articles/07369 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b7ea2dc5b561868248bd11761649e432 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
|---|