Protein oligomer structure prediction using GALAXY in CASP14
العنوان: | Protein oligomer structure prediction using GALAXY in CASP14 |
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المؤلفون: | Chaok Seok, Hyeonuk Woo, Jinsol Yang, Taeyong Park, Jonghun Won, Sohee Kwon |
المصدر: | Proteins: Structure, Function, and Bioinformatics. 89:1844-1851 |
بيانات النشر: | Wiley, 2021. |
سنة النشر: | 2021 |
مصطلحات موضوعية: | Models, Molecular, chemistry.chemical_classification, Protein Folding, Materials science, Protein Conformation, Biomolecule, Protein subunit, Ab initio, Computational Biology, Proteins, Protein structure prediction, Biochemistry, Oligomer, Molecular Docking Simulation, Protein Subunits, chemistry.chemical_compound, Monomer, Protein structure, chemistry, Sequence Analysis, Protein, Structural Biology, Docking (molecular), Biological system, Molecular Biology, Software |
الوصف: | Proteins perform their functions by interacting with other biomolecules. For these interactions, proteins often form homo- or hetero-oligomers as well. Thus, oligomer protein structures provide important clues regarding the biological roles of proteins. To this end, computational prediction of oligomer structures may be a useful tool in the absence of experimentally resolved structures. Here, we describe our server and human-expert methods used to predict oligomer structures in the CASP14 experiment. Examples are provided for cases in which manual domain-splitting led to improved oligomeric domain structures by ab initio docking, automated oligomer structure refinement led to improved subunit orientation and terminal structure, and manual oligomer modeling utilizing literature information generated a reasonable oligomer model. We also discussed the results of post-prediction docking calculations with AlphaFold2 monomers as input in comparison to our blind prediction results. Overall, ab initio docking of AlphaFold2 models did not lead to better oligomer structure prediction, which may be attributed to the interfacial structural difference between the AlphaFold2 monomer structures and the crystal oligomer structures. This result poses a next-stage challenge in oligomer structure prediction after the success of AlphaFold2. For successful protein assembly structure prediction, a different approach that exploits further evolutionary information on the interface and/or flexible docking taking the interfacial conformational flexibilities of subunit structures into account is needed. |
تدمد: | 1097-0134 0887-3585 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7fc3b4f4bce98a3680bd03dc9869e59 https://doi.org/10.1002/prot.26203 |
حقوق: | CLOSED |
رقم الأكسشن: | edsair.doi.dedup.....b7fc3b4f4bce98a3680bd03dc9869e59 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 10970134 08873585 |
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