Antibody surface coverage drives matrix interference in microfluidic capillary immunoassays
| العنوان: | Antibody surface coverage drives matrix interference in microfluidic capillary immunoassays |
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| المؤلفون: | Alexander D. Edwards, Ana I. Barbosa, Nuno M. Reis |
| المصدر: | ACS Sensors Barbosa, A I, Edwards, A D & Reis, N M 2021, ' Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays ', ACS Sensors, vol. 6, no. 7, pp. 2682-2690 . https://doi.org/10.1021/acssensors.1c00704 |
| بيانات النشر: | American Chemical Society, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Capillary action, Microfluidics, microfluidics, Bioengineering, 02 engineering and technology, Biosensing Techniques, 01 natural sciences, Article, Antibodies, Matrix (chemical analysis), SDG 3 - Good Health and Well-being, Interference (communication), medicine, Sample dilution, Instrumentation, matrix effect, Fluid Flow and Transfer Processes, Immunoassay, Chromatography, medicine.diagnostic_test, biology, Chemistry, Process Chemistry and Technology, 010401 analytical chemistry, protein biomarkers, 021001 nanoscience & nanotechnology, biosensors, 0104 chemical sciences, microcapillary film, Point-of-Care Testing, biology.protein, Antibody, 0210 nano-technology, Biosensor |
| الوصف: | The performance of biosensors is often optimized in buffers, which brings inconsistencies during applications with biological samples. Current strategies for minimizing sample (matrix) interference are complex to automate and miniaturize, involving, e.g., sample dilution or recovery of serum/plasma. This study shows the first systematic analysis using hundreds of actual microfluidic immunoassay fluoropolymer strips to understand matrix interference in microflow systems. As many interfering factors are assay-specific, we have explored matrix interference for a range of enzymatic immunoassays, including a direct mIgG/anti-mIgG, a sandwich cancer biomarker PSA, and a sandwich inflammatory cytokine IL-1β. Serum matrix interference was significantly affected by capillary antibody surface coverage, suggesting for the first time that the main cause of the serum matrix effect is low-affinity serum components (e.g., autoantibodies) competing with high-affinity antigens for the immobilized antibody. Additional experiments carried out with different capillary diameters confirmed the importance of antibody surface coverage in managing matrix interference. Building on these findings, we propose a novel analytical approach where antibody surface coverage and sample incubation times are key for eliminating and/or minimizing serum matrix interference, consisting in bioassay optimization carried out in serum instead of buffer, without compromising the performance of the bioassay or adding extra cost or steps. This will help establishing a new route toward faster development of modern point-of-care tests and effective biosensor development. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2379-3694 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b862a57315ef9168286dd75942a4e1b1 https://centaur.reading.ac.uk/98925/9/acssensors.1c00704.pdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b862a57315ef9168286dd75942a4e1b1 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 23793694 |
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