Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy
| العنوان: | Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy |
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| المؤلفون: | Roger Riera, Jana Tauler, Natàlia Feiner‐Gracia, Salvador Borrós, Cristina Fornaguera, Lorenzo Albertazzi |
| المساهمون: | Nanoscopy for Nanomedicine, Molecular Biosensing for Med. Diagnostics, ICMS Core |
| المصدر: | ChemMedChem, 17(13):e202100633. Wiley-VCH Verlag Dipòsit Digital de la UB Universidad de Barcelona |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Pharmacology, Microscopy, Organic Chemistry, Green Fluorescent Proteins, direct stochastic optical reconstruction microscopy (dSTORM), Transfection, SDG 3 – Goede gezondheid en welzijn, Microscòpia mèdica, Biochemistry, Nanomedicine, SDG 3 - Good Health and Well-being, Drug Discovery, Nanomedicina, Medical microscopy, Molecular Medicine, Nanoparticles, cell trafficking, Pèptids, General Pharmacology, Toxicology and Pharmaceutics, poly(β-aminoester) nanoparticles, Peptides, Oligopeptides, nanoparticle stability |
| الوصف: | Nanomedicine emerged some decades ago with the hope to be the solution for most unmet medical needs. However, tracking materials at nanoscale is challenging to their reduced size, below the resolution limit of most conventional techniques. In this context, we propose the use of direct stochastic optical reconstruction microscopy (dSTORM) to study time stability and cell trafficking after transfection of oligopeptide end-modified poly(β-aminoester) (OM-pBAE) nanoparticles. We selected different combinations of cationic end oligopeptides (arginine – R; histidine – H; and lysine – K) among polymer libraries, since the oligopeptide combination demonstrated to be useful for different applications, such as vaccination and gene silencing. We demonstrate that their time evolution as well as their cell uptake and trafficking are dependent on the oligopeptide. This study opens the pave to broad mechanistic studies at nanoscale that could enable a rational selection of specific pBAE nanoparticles composition after determining their stability and cell trafficking. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1860-7179 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9178cd4e359339b79f4930c77a6b607 http://hdl.handle.net/2445/188882 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b9178cd4e359339b79f4930c77a6b607 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18607179 |
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