Role of Serum Amyloid A in Adipocyte-Macrophage Cross Talk and Adipocyte Cholesterol Efflux
| العنوان: | Role of Serum Amyloid A in Adipocyte-Macrophage Cross Talk and Adipocyte Cholesterol Efflux |
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| المؤلفون: | Adeline Divoux, Stéphane Commans, Joan Tordjman, Abdelhaim Aissat, Aurélie Faty, Christine Poitou, Mayoura Keophiphath, Karine Clément, Corneliu Henegar, Danielle Hugol |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism. 94:1810-1817 |
| بيانات النشر: | The Endocrine Society, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Microarray, Adipose Tissue, White, Endocrinology, Diabetes and Metabolism, Cellular differentiation, Clinical Biochemistry, Adipose tissue, Cell Communication, Biology, Biochemistry, Proinflammatory cytokine, chemistry.chemical_compound, Endocrinology, Internal medicine, Adipocyte, Adipocytes, medicine, Humans, Serum amyloid A, Serum Amyloid A Protein, Reverse Transcriptase Polymerase Chain Reaction, Microarray analysis techniques, Macrophages, Biochemistry (medical), Cell Differentiation, Immunohistochemistry, Culture Media, Obesity, Morbid, stomatognathic diseases, Cholesterol, chemistry, Culture Media, Conditioned, Female, Lipoprotein |
| الوصف: | Acute phase serum amyloid A (A-SAA) is secreted by hepatocytes in response to injury and is regulated by proinflammatory cytokines. In obese humans, adipocytes are also a major contributor to circulating A-SAA levels.We aimed to investigate the role and regulation of A-SAA in human adipose tissue (AT).An approach combining microarrays and the FunNet bioinformatics tool was applied to human AT fractions (i.e. adipocytes vs. stroma vascular fraction) to hypothesize genes and functions related to A-SAA. Experiments with human AT from 37 obese subjects and human multipotent adipose-derived stem (hMADS) cells were used to confirm the microarray driven hypotheses.Microarray analysis highlighted the relationship between A-SAA and stroma vascular fraction inflammatory genes, and between A-SAA and adipocyte-expressed ATP-binding cassette (ABC) transporters. We confirmed that serum amyloid A (SAA) protein is expressed in sc AT of obese subjects (n = 37, body mass index = 49.3 +/- 1.5 kg/m(2)) and showed that SAA protein expression correlated with adipocyte size (R = 0.44; P = 6.10(-3)), macrophage infiltration (R = 0.61; P = 10(-4)), and ABC subfamily A1 protein expression (R = 0.43; P = 9.10(-3)). IL-1beta, TNF-alpha, and human AT macrophage-conditioned medium significantly induced A-SAA secretion (from 2.6 to 7.6 fold) in hMADS cells. Recombinant SAA induced cholesterol ABC subfamily A1-dependent efflux from hMADS adipocytes by 4.3-fold in a dose-dependent manner.This work provides original insight suggesting that A-SAA is a player in the dialogue between hypertrophied adipocytes and macrophages through its regulation of adipocyte cholesterol efflux. |
| تدمد: | 1945-7197 0021-972X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9b55ce59aaccfba35cc7643c2199e61 https://doi.org/10.1210/jc.2008-2040 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....b9b55ce59aaccfba35cc7643c2199e61 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457197 0021972X |
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