Linalool and eugenol exhibit apoptotic potential on hela and caco-2 cells through the modulation of src kinases and ADAMTS proteases while only eugenol displays anti-angiogenic features on HeLa cells
| العنوان: | Linalool and eugenol exhibit apoptotic potential on hela and caco-2 cells through the modulation of src kinases and ADAMTS proteases while only eugenol displays anti-angiogenic features on HeLa cells |
|---|---|
| المؤلفون: | Mehmet Ali Kisacam, Fatih Sakin, Sevgi Irtegun-Kandemir, Gulsum Pektanc-Sengul, Cemil Kurekci |
| المساهمون: | Dicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalı, İrtegün-Kandemir, Sevgi |
| المصدر: | Biologia. 77:865-877 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cancer cells, Eugenol, Genetics, ADAMTS, Animal Science and Zoology, Cell Biology, Plant Science, Cellular Apoptosis, Molecular Biology, Biochemistry, Linalool, Ecology, Evolution, Behavior and Systematics, Src Kinase |
| الوصف: | The cytotoxic effects of linalool and eugenol on HeLa and Caco-2 cancer cells were investigated. The expression of the Fyn, Hck, and ADAMTS genes and Bcl-2, Bax, VEGF proteins were determined through the real-time-qPCR and Western blot analysis, respectively. IC50 values of linalool on HeLa cell were 799.6, 1228, and 1239 mu g/mL after 24, 48, and 72 h, respectively, whereas those of eugenol were 388, 94.35, and 73.78 mu g/mL. IC50 values in Caco-2 cells were 887.7, 1259, and 1356 mu g/mL for linalool whereas those of eugenol were 452.7, 578.3, and 433.3 mu g/mL after 24, 48, and 72 h, respectively. As a control, Hek-293 IC50 values were 1047 and 556.7 mu g/mL for linalool and eugenol, respectively. Linalool at 1024 mu g/mL concentration increased Bax and Bcl-2 levels significantly in both cell lines (p < 0.001). However, eugenol treatment did not alter Bax levels (p > 0.05), but it decreased Bcl-2 levels significantly (p < 0.001). Additionally, 1024 mu g/mL linalool exposure increased Fyn gene expression in Caco-2 cells, which was not observed for HeLa cells. Unlike linalool, eugenol upregulated ADAMTS1 gene expression and decreased VEGF levels in HeLa cells. On the contrary, in Caco-2 cells, eugenol treatment did not affect ADAMTS1 levels, whilst linalool upregulated ADAMTS1 levels, especially at 1024 mu g/mL concentration. VEGF levels increased in Caco-2 cells following eugenol treatment. ADAMTS3 levels decreased in Caco-2 cells with exception of 1024 mu g/mL linalool treatment. These results indicated the anti-proliferative, apoptotic effects of plant-derived natural compounds. It is also revealed that eugenol might also display antiangiogenic properties on HeLa cells in addition to its apoptotic effects. |
| وصف الملف: | application/pdf |
| تدمد: | 1336-9563 0006-3088 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9c44612cdaaa06051f57c7d4e5fea0a https://doi.org/10.1007/s11756-021-00991-8 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....b9c44612cdaaa06051f57c7d4e5fea0a |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 13369563 00063088 |
|---|