Expansion of tropism of a feline parvovirus to target a human tumor cell line by display of an αv integrin binding peptide on the capsid
| العنوان: | Expansion of tropism of a feline parvovirus to target a human tumor cell line by display of an αv integrin binding peptide on the capsid |
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| المؤلفون: | J.T. Chapman, AL Spitzer, Sebastian Leptihn, Françoise Maxwell, Ian H. Maxwell, JA Corsini, L.C. Scherrer |
| المصدر: | Gene Therapy. 8:324-331 |
| بيانات النشر: | Springer Science and Business Media LLC, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Keratinocytes, Skin Neoplasms, viruses, Integrin, Gene Expression, Breast Neoplasms, Integrin alpha5, Feline panleukopenia, Recombinant virus, Polymerase Chain Reaction, Virus, Transduction (genetics), Capsid, Antigens, CD, Transduction, Genetic, Rhabdomyosarcoma, Tumor Cells, Cultured, Genetics, Humans, Luciferases, Melanoma, Molecular Biology, Ovarian Neoplasms, biology, Parvovirus, Liver Neoplasms, Genetic Therapy, biology.organism_classification, Virology, Oncolytic virus, Gene Targeting, Carcinoma, Squamous Cell, biology.protein, Molecular Medicine, Female, Endothelium, Vascular, Feline Panleukopenia Virus, Carrier Proteins |
| الوصف: | The autonomous parvoviruses are small, non-enveloped, single strand DNA viruses. They occur in many species and they have oncolytic properties. We are modifying the capsid of feline panleukopenia virus (FPV), a parvovirus which normally infects feline cells, with the goal of targeting human tumor cells for potential cancer therapy. Using recombinant viruses transducing a luciferase reporter, we show that insertion of a cyclically constrained, integrin-binding peptide at an exposed position on the FPV capsid enables transduction of an alpha(v) integrin-expressing human rhabdomyosarcoma cell line (Rh18A). These cells were not transduced by virus with the unmodified FPV capsid. Transduction of Rh18A was specifically inhibited by an alpha(v) integrin blocking antibody. However, other human tumor lines expressing alpha(v) integrins were not transduced by virus with either the modified or unmodified capsid. We conclude that modification of the FPV capsid to bind alpha(v) integrins can contribute to, but is not generally sufficient for, redirecting infection to human tumor cells. The permissiveness of Rh18A cells presumably involves additional factors unique to this line among various human cell lines tested. |
| تدمد: | 1476-5462 0969-7128 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba06798affee41f2cc8ec446b54f831e https://doi.org/10.1038/sj.gt.3301399 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ba06798affee41f2cc8ec446b54f831e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765462 09697128 |
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