Immunohistochemical characterisation of the hepatic stem cell niche in feline hepatic lipidosis: a preliminary morphological study
| العنوان: | Immunohistochemical characterisation of the hepatic stem cell niche in feline hepatic lipidosis: a preliminary morphological study |
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| المؤلفون: | Valtolina, Chiara, Robben, Joris H, Favier, Robert P, Rothuizen, Jan, Grinwis, Guy Cm, Schotanus, Baukje A, Penning, Louis C, Veterinair Pathologisch Diagnostisch Cnt, dPB CR, dCSCA AVR, Intensieve Zorgafdeling, Sub Hepatologie, LS Interne geneeskunde, Onderzoek, dCSCA RMSC-1 |
| المساهمون: | Veterinair Pathologisch Diagnostisch Cnt, dPB CR, dCSCA AVR, Intensieve Zorgafdeling, Sub Hepatologie, LS Interne geneeskunde, Onderzoek, dCSCA RMSC-1 |
| المصدر: | Journal of Feline Medicine and Surgery, 21(2), 165. W.B. Saunders Ltd Journal of Feline Medicine and Surgery |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Feline hepatic lipidosis, Liver chemistry, Pathology, medicine.medical_specialty, Stromal cell, 040301 veterinary sciences, Biology, Cat Diseases, 030308 mycology & parasitology, Hepatic stem cell, 0403 veterinary science, 03 medical and health sciences, medicine, Animals, Stem Cell Niche, Progenitor cell, Small Animals, 0303 health sciences, CATS, Original Articles, 04 agricultural and veterinary sciences, medicine.disease, Immunohistochemistry, Staining, Fatty Liver, Liver, Cats |
| الوصف: | Objectives The aim of this study was to describe the cellular and stromal components of the hepatic progenitor cell niche in feline hepatic lipidosis (FHL). Methods Immunohistochemical staining for the progenitor/bile duct marker (K19), activated Kupffer cells (MAC387), myofibroblasts (alpha-smooth muscle actin [α-SMA]) and the extracellular matrix component laminin were used on seven liver biopsies of cats with FHL and three healthy cats. Double immunofluorescence stainings were performed to investigate co-localisation of different cell types in the hepatic progenitor cell (HPC) niche. Results HPCs, Kupffer cells, myofibroblasts and laminin deposition were observed in the liver samples of FHL, although with variability in the expression and positivity of the different immunostainings between different samples. When compared with the unaffected cats where K19 positivity and minimal α-SMA and laminin positivity were seen mainly in the portal area, in the majority of FHL samples K19 and α-SMA-positive cells and laminin positivity were seen also in the periportal and parenchymatous area. MAC387-positive cells were present throughout the parenchyma. Conclusions and relevance This is a preliminary morphological study to describe the activation and co-localisation of components of the HPC niche in FHL. Although the HPC niche in FHL resembles that described in hepatopathies in dogs and in feline lymphocytic cholangitis, the expression of K19, α-SMA, MAC387 and lamin is more variable in FHL, and a common pattern of activation could not be established. Nevertheless, when HPCs were activated, a spatial association between HPCs and their niche could be demonstrated. |
| وصف الملف: | image/pdf |
| اللغة: | English |
| تدمد: | 1098-612X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba34ab6e3257568efb0ba516ed82fab6 https://dspace.library.uu.nl/handle/1874/380069 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....ba34ab6e3257568efb0ba516ed82fab6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098612X |
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