Enteroviruses in blood of patients with type 1 diabetes detected by integrated cell culture and reverse transcription quantitative real-time PCR
| العنوان: | Enteroviruses in blood of patients with type 1 diabetes detected by integrated cell culture and reverse transcription quantitative real-time PCR |
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| المؤلفون: | Christine Lefevre, Ilham Moumna, Marie-Christine Vantyghem, Famara Sane, Ilka Engelmann, Enagnon Kazali Alidjinou, Agathe Baras, Didier Hober |
| المصدر: | Acta Diabetologica. 54:1025-1029 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Adolescent, Endocrinology, Diabetes and Metabolism, 030106 microbiology, Coxsackievirus, Real-Time Polymerase Chain Reaction, medicine.disease_cause, Sensitivity and Specificity, Leukocyte Count, Young Adult, 03 medical and health sciences, Endocrinology, Enterovirus Infections, Leukocytes, Internal Medicine, medicine, Humans, Child, Cells, Cultured, Enterovirus, biology, Reverse Transcriptase Polymerase Chain Reaction, RNA, General Medicine, Middle Aged, biology.organism_classification, Virology, Molecular biology, Reverse transcriptase, Diabetes Mellitus, Type 1, 030104 developmental biology, Real-time polymerase chain reaction, Cell culture, Vero cell, RNA, Viral, Female, Viral load, Blood Chemical Analysis |
| الوصف: | Enteroviruses (EV) have been associated with type 1 diabetes (T1D), but EV RNA detection has been reported in only a small proportion of T1D patients. We studied whether integrated cell culture and reverse transcription real-time PCR could improve EV detection in blood samples from patients with T1D. Blood was collected from 13 patients with T1D. The presence of EV RNA in blood was investigated by using real-time RT-PCR. In addition, plasma and white blood cells (WBC) were inoculated to BGM and Vero cell line cultures. Culture supernatants and cells collected on day 7 and day 14 were tested for EV RNA by real-time RT-PCR. Enterovirus identification was performed through sequencing of the VP4/VP2 region. Enterovirus RNA was detected in blood by using real-time RT-PCR in only one out of 13 patients. The detection of EV RNA in cultures inoculated with clinical samples (plasma and/or WBC) gave positive results in five other patients. The viral loads were low, ranging from 45 to 4420 copies/ng of total RNA. One isolate was successfully identified as coxsackievirus B1. Integrated cell culture and reverse transcription real-time PCR can improve the detection rate of EV in blood samples of patients with T1D and can be useful to investigate further the relationship between EV and the disease. |
| تدمد: | 1432-5233 0940-5429 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba3f83c381b9d058afe84878ea590c13 https://doi.org/10.1007/s00592-017-1041-7 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....ba3f83c381b9d058afe84878ea590c13 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14325233 09405429 |
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